A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

A method of preparing a composition comprising mycelium along with a cellulose and/or plurality of nanoparticles is described herein. The method comprises inoculating a liquid medium with fungus, the liquid medium comprising nutrients and the cellulose and/or nanoparticles. Further described herein are compositions comprising mycelium along with a cellulose and/or plurality of nanoparticles, as well as articles-of-manufacture comprising such a composition, wherein at least 10% of the cellulose and/or nanoparticles in the composition is incorporated within the mycelium. A method of enhancing fungal growth is also described, comprising contacting the fungus with a liquid medium comprising a polymer which comprises carboxylic acid groups.

An inoculation apparatus is provided. The inoculation apparatus includes a vessel for containing liquid mycelium culture having: an outlet port; an injection port; and a breather port. The inoculation apparatus also includes an auto-fill syringe having a nozzle and an inlet port; a connection line with a first end connecting to the outlet port and a second end connecting to the inlet port; and an injection means connected to the nozzle. Related methods are also provided.

A process of making a mycological biopolymer of a mycomaterial filter comprising the steps of filling a scaffold with a nutritive substrate and a fungus, placing an encasement on the scaffold to seal the scaffold, said encasement having only one outlet therein open to fresh air and defining a vacant space, incubation of the sealed scaffold at high temperatures and carbon dioxide concentrations to induce biopolymer growth into the vacant space wherein the mycological biopolymer environmental conditions comprise an environmental temperature from 55° F. to 95° F. and carbon dioxide constitutes from 2% to 8% of the environment within the vacant space, and thereafter drying the produced mycological biopolymer.

A method for cultivating ergosterol-rich Agaricus bisporus includes sowing seeds on a culture medium followed by soil covering for mycelium cultivation and sporophore cultivation. During the mycelium cultivation, a light intensity is 100-400 lux and a pH of the culture medium is controlled to 6.0-8.0. During the sporophore cultivation, a light intensity is 20-200 lux and a pH of the culture medium is controlled to 6.0-8.0. The preparation of the culture medium includes the following steps. The manure is mixed with phosphate fertilizer, calcium fertilizer and nitrogen fertilizer to obtain an auxiliary material, and the crop straw and the auxiliary material are subjected to composting and decomposition, pasteurized and adjusted pH to 6.8-7.2 to obtain the culture medium. A healthy food or a functional food containing the ergosterol-rich Agaricus bisporus is also provided herein.

A method for cultivating ergosterol-rich Agaricus bisporus includes sowing seeds on a culture medium followed by soil covering for mycelium cultivation and sporophore cultivation. During the mycelium cultivation, a light intensity is 100-400 lux and a pH of the culture medium is controlled to 6.0-8.0. During the sporophore cultivation, a light intensity is 20-200 lux and a pH of the culture medium is controlled to 6.0-8.0. The preparation of the culture medium includes the following steps. The manure is mixed with phosphate fertilizer, calcium fertilizer and nitrogen fertilizer to obtain an auxiliary material, and the crop straw and the auxiliary material are subjected to composting and decomposition, pasteurized and adjusted pH to 6.8-7.2 to obtain the culture medium. A healthy food or a functional food containing the ergosterol-rich Agaricus bisporus is also provided herein.

A mycelium growth bed for optimal production of pure mycelium or a pure mycelium composite with controlled or predictable properties, the bed comprising a tray, a conveying platform, a permeable membrane, a substrate, and a porous material. The permeable membrane is positioned on the conveying platform within the tray. The substrate is positioned on the permeable membrane and the porous material is positioned on top of the substrate. The system provides a configuration wherein the CO.sub.2 concentration is held above 3%, the relative humidity is held above 40% and the O.sub.2 concentration is held below 20% in steady state conditions to produce leather-like mycelium without fruiting bodies.

A mycelium growth bed for optimal production of pure mycelium or a pure mycelium composite with controlled or predictable properties, the bed comprising a tray, a conveying platform, a permeable membrane, a substrate, and a porous material. The permeable membrane is positioned on the conveying platform within the tray. The substrate is positioned on the permeable membrane and the porous material is positioned on top of the substrate. The system provides a configuration wherein the CO.sub.2 concentration is held above 3%, the relative humidity is held above 40% and the O.sub.2 concentration is held below 20% in steady state conditions to produce leather-like mycelium without fruiting bodies.

Disclosed is a chemical-physical method of producing mushrooms enriched with biologically active substances including steps of isolation and pre-seeding development of the mycelium of a desired fungal species, maturation of the substrate, mycelium sowing and incubation with development of fungal primordia under conditions controlled and assisted by machines and processors designed to adjust temperature, humidity and radiation, development of carpophores, their collection and treatment with pulsed light in order to stimulate the production of vitamin D2. Physical treatment includes exposing the environment to electromagnetic fields (EMF) during all the previous steps the collection, and chemical treatment includes adding the substrate with egg powder. After the mycelium sowing, the substrate is enriched with inorganic trace elements, with a result of obtaining officinal mushrooms that are provided with therapeutic capacities in particular in oncology field and, in general, adapted to counter cell degeneration in a variety of diseases.