A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

A method of preparing a high yield of mushroom β-glucan is provided. The method includes: providing a liquid culture to culture the mushroom mycelium by fermentation, to increase the yields of the mushroom mycelium and polysaccharide, wherein the liquid culture comprises at least two ingredients selected from the groups consisting of glucose, trehalose, a dietary fiber and mannose or derivatives thereof; and rupturing the mushroom mycelium with a continuous multiple-ultrasonic equipment; and removing insoluble matters from the liquid culture. A method of preparing highly pure mushroom β-glucan powder and solution and the products thereof are also provided. By the method of the present disclosure, the yield of mushroom β-glucan is effectively increased, its activity loss is reduced, and the stability of product thereof is improved.

Disclosed is a chemical-physical method of producing mushrooms enriched with biologically active substances including steps of isolation and pre-seeding development of the mycelium of a desired fungal species, maturation of the substrate, mycelium sowing and incubation with development of fungal primordia under conditions controlled and assisted by machines and processors designed to adjust temperature, humidity and radiation, development of carpophores, their collection and treatment with pulsed light in order to stimulate the production of vitamin D2. Physical treatment includes exposing the environment to electromagnetic fields (EMF) during all the previous steps the collection, and chemical treatment includes adding the substrate with egg powder. After the mycelium sowing, the substrate is enriched with inorganic trace elements, with a result of obtaining officinal mushrooms that are provided with therapeutic capacities in particular in oncology field and, in general, adapted to counter cell degeneration in a variety of diseases.

A planting method for morels is disclosed, including the following steps of: (1) preparing spawns; (2) flipping the spawns; (3) managing the humidity; and (4) fruiting for harvesting. The planting method is simple, is easy to learn and promote, has a high yield, and requires few spawns, thereby reducing planting costs and product costs.

A planting method for morels is disclosed, including the following steps of: (1) preparing spawns; (2) flipping the spawns; (3) managing the humidity; and (4) fruiting for harvesting. The planting method is simple, is easy to learn and promote, has a high yield, and requires few spawns, thereby reducing planting costs and product costs.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material.

The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is a made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material. In another embodiment, the fungus is allowed to grow as a fruiting body out of the substrate and within an enclosure to completely fill the enclosure to form a self-supporting structure.

The present disclosure provides a method for aseptically culturing fruiting bodies of Antrodia cinnamomea, including: inoculating Antrodia cinnamomea strains in a sterilized agar medium, and performing a subculture to generate secondary mycelia and fruiting bodies of Antrodia cinnamomea.

The method grows a mycelial mass over a three-dimensional lattice such that a dense network of oriented hyphae is formed on the lattice. Growth along the lattice results in mycelium composite with highly organized hyphae strands and allows the design and production of composites with greater strength in chosen directions due to the organized nature of the supporting mycelia structure.