The product is made, in part, of a network of interconnected mycelia cells forming a mass. In one embodiment, the mass includes one or more embedded elements, such as a panel. In another embodiment, the mass is formed over a three-dimensional lattice. The mycelia cells form hyphae that bond directly to panels made of cellulosic materials.

A monokaryotic mycelium sheet producing system for creating a sheet of monokaryotic mycelial material. The mycelium sheet producing system includes a culture unit, a spore stock unit, a plating unit, a section unit, a sub-plating unit, an expanding unit and a colonization unit. The culture unit prepares a monokaryon culture. The spore stock unit grows a plurality of fruit bodies in sterile laboratory conditions to create a spore stock. The plating unit performs a peroxide-based spore rescue and a plating process. The section unit is adaptable to section robust hyphae. The sub-plating unit sub-plates and expands the robust hyphae onto a spawn grain master. The expanding unit subsequently expands the spawn grain master into appropriate production of spawn volume. The colonization unit is adaptable to perform a subsequent colonization of mycelium substrate thereby creating a substantially defect free sheet of mycelium.

The method grows a mycelial mass over a three-dimensional lattice such that a dense network of oriented hyphae is formed on the lattice. Growth along the lattice results in mycelium composite with highly organized hyphae strands and allows the design and production of composites with greater strength in chosen directions due to the organized nature of the supporting mycelia structure.

The method grows a mycelial mass over a three-dimensional lattice such that a dense network of oriented hyphae is formed on the lattice. Growth along the lattice results in mycelium composite with highly organized hyphae strands and allows the design and production of composites with greater strength in chosen directions due to the organized nature of the supporting mycelia structure.

The present disclosure provides a cultivation method of morels and belongs to the technical field of cultivation of edible fungi. After nutrient contents that can be metabolized and utilized by morel mycelia are scientifically matched, sterilization treatment of exogenous nutrients is eliminated, and the exogenous nutrients are directly compressed for forming. Formed exogenous nutrient blocks and spawn is sowed and covered with soil, so as to realize a potential difference between rich and poor nutrients in a physical space. When the spawn germinate to form a mycelium network, the mycelia will enter a nutrient-rich exogenous nutrient area, secrete various extracellular enzymes to decompose and utilize these exogenous nutrient blocks, and transport them to a nutrient-poor mycelium network for storage, so as to complete nutrient assimilation and absorption, and reserve energy for later sexual reproduction.

The present disclosure provides a cultivation method of morels and belongs to the technical field of cultivation of edible fungi. After nutrient contents that can be metabolized and utilized by morel mycelia are scientifically matched, sterilization treatment of exogenous nutrients is eliminated, and the exogenous nutrients are directly compressed for forming. Formed exogenous nutrient blocks and spawn is sowed and covered with soil, so as to realize a potential difference between rich and poor nutrients in a physical space. When the spawn germinate to form a mycelium network, the mycelia will enter a nutrient-rich exogenous nutrient area, secrete various extracellular enzymes to decompose and utilize these exogenous nutrient blocks, and transport them to a nutrient-poor mycelium network for storage, so as to complete nutrient assimilation and absorption, and reserve energy for later sexual reproduction.

A controlled growth system is provided herein. The controlled growth system includes a controlled growth environment, a controller, a sensor, and a computing system. The controlled growth environment is configured to grow a biologic. The controller is in communication with the controlled growth environment. The controller is configured to manage process parameters of the controlled growth environment. The sensor is configured to monitor the biologic during a growth process. The computing system is in communication with the sensor and the controller. The computing system is programmed to perform operations for achieving a desired final quality metric for the biologic.

A method of preparing a high yield of mushroom -glucan is provided. The method includes: providing a liquid culture to culture the mushroom mycelium by fermentation, to increase the yields of the mushroom mycelium and polysaccharide, wherein the liquid culture comprises at least two ingredients selected from the groups consisting of glucose, trehalose, a dietary fiber and mannose or derivatives thereof; and rupturing the mushroom mycelium with a continuous multiple-ultrasonic equipment; and removing insoluble matters from the liquid culture. A method of preparing highly pure mushroom -glucan powder and solution and the products thereof are also provided. By the method of the present disclosure, the yield of mushroom -glucan is effectively increased, its activity loss is reduced, and the stability of product thereof is improved.

Disclosed is a chemical-physical method of producing mushrooms enriched with biologically active substances including steps of isolation and pre-seeding development of the mycelium of a desired fungal species, maturation of the substrate, mycelium sowing and incubation with development of fungal primordia under conditions controlled and assisted by machines and processors designed to adjust temperature, humidity and radiation, development of carpophores, their collection and treatment with pulsed light in order to stimulate the production of vitamin D2. Physical treatment includes exposing the environment to electromagnetic fields (EMF) during all the previous steps the collection, and chemical treatment includes adding the substrate with egg powder. After the mycelium sowing, the substrate is enriched with inorganic trace elements, with a result of obtaining officinal mushrooms that are provided with therapeutic capacities in particular in oncology field and, in general, adapted to counter cell degeneration in a variety of diseases.

The use of particular terpenes, or of essential oils that are mainly composed thereof, as biostimulants in the production of mycelia and the cultivation of mushrooms. More particular, the invention relates to the use of at least one beta-pinene, delta-3-carene or beta-phellandrene chemotyped essential oil, or of at least one terpene selected from beta-pinene, delta-3-carene or beta-phellandrene, or one of the mixtures thereof, for stimulating the development and growth of mycelia and of edible mushrooms.