A pasture grass substrate includes a dry material and water. The dry material includes pasture grasses, corncobs and rice brans. The weight ratio of the pasture grasses in the dry material is from 55% to 75%. The weight ratio of the corncobs in the dry material is from 20% to 30%. The weight ratio of the rice brans in the dry material is from 5% to 15%. The incubating methods of mushrooms are also disclosed.

A pasture grass substrate includes a dry material and water. The dry material includes pasture grasses, corncobs and rice brans. The weight ratio of the pasture grasses in the dry material is from 55% to 75%. The weight ratio of the corncobs in the dry material is from 20% to 30%. The weight ratio of the rice brans in the dry material is from 5% to 15%. The incubating methods of mushrooms are also disclosed.

A planting method for morels is disclosed, including the following steps of: (1) preparing spawn; (2) flipping the spawn; (3) managing the humidity; and (4) fruiting for harvesting. The planting method is simple, is easy to learn and promote, has a high yield, and requires few spawn, thereby reducing planting costs and product costs.

A planting method for morels is disclosed, including the following steps of: (1) preparing spawn; (2) flipping the spawn; (3) managing the humidity; and (4) fruiting for harvesting. The planting method is simple, is easy to learn and promote, has a high yield, and requires few spawn, thereby reducing planting costs and product costs.

An inoculation apparatus is provided. The inoculation apparatus includes a vessel for containing liquid mycelium culture having: an outlet port; an injection port; and a breather port. The inoculation apparatus also includes an auto-fill syringe having a nozzle and an inlet port; a connection line with a first end connecting to the outlet port and a second end connecting to the inlet port; and an injection means connected to the nozzle. Related methods are also provided.

An inoculation apparatus is provided. The inoculation apparatus includes a vessel for containing liquid mycelium culture having: an outlet port; an injection port; and a breather port. The inoculation apparatus also includes an auto-fill syringe having a nozzle and an inlet port; a connection line with a first end connecting to the outlet port and a second end connecting to the inlet port; and an injection means connected to the nozzle. Related methods are also provided.

Disclosed is a chemical-physical method of producing mushrooms enriched with biologically active substances including steps of isolation and pre-seeding development of the mycelium of a desired fungal species, maturation of the substrate, mycelium sowing and incubation with development of fungal primordia under conditions controlled and assisted by machines and processors designed to adjust temperature, humidity and radiation, development of carpophores, their collection and treatment with pulsed light in order to stimulate the production of vitamin D2. Physical treatment includes exposing the environment to electromagnetic fields (EMF) during all the previous steps the collection, and chemical treatment includes adding the substrate with egg powder. After the mycelium sowing, the substrate is enriched with inorganic trace elements, with a result of obtaining officinal mushrooms that are provided with therapeutic capacities in particular in oncology field and, in general, adapted to counter cell degeneration in a variety of diseases.

Disclosed is a method to prepare a myceliated high-protein food product with increased digestibility, decreased phytic acid component, decreased oryzacystatin, and/or increased polyphenol content, which includes culturing a fungi an aqueous media which has a high level of plant protein, for example at least 20 g protein per 100 g dry weight with excipients, on a dry weight basis. The plant protein can include pea, rice and/or chickpea. The fungi can include comprises Lentinula spp., Agaricus spp., Pleurotus spp., Boletus spp., or Laetiporus spp. After culturing, the material is harvested by obtaining the myceliated high-protein food product via drying or concentrating. The resultant myceliated high-protein food product may have its taste, flavor, or aroma modulated, such as by increasing desirable flavors or tastes such as meaty, savory, umami, popcorn and/or by decreasing undesirable flavors such as bitterness, astringency or beaniness. Deflavoring and/or deodorizing as compared to non-myceliated control materials can also be observed. Also disclosed are myceliated high-protein food products made by e.g. the methods of the invention. Foods such as textured protein, dairy analogs, crisps, and the like may include the high protein food products disclosed.

A planting method for morels is disclosed, including the following steps of: (1) preparing spawns; (2) flipping the spawns; (3) managing the humidity; and (4) fruiting for harvesting. The planting method is simple, is easy to learn and promote, has a high yield, and requires few spawns, thereby reducing planting costs and product costs.

The invention is a method for enhanced growth of mycelium in culture including the key steps of seed blend choice from among corn, rice, quinoa, chia, canihua, cumin and flax seed, germinating the seed blend prior to inoculating the mycelium starter, and conducting the mushroom cultivation with water and particular transmissions of sound and particularly colored light. Mushrooms grown by such a method not only mature in 13-15 days rather than 21 days, but have an enhanced nutritional and medicinal profile along with absence of unwanted organism contaminants whose unwanted overgrowth occurs during days 16-21 (or days 16-28) of typical mushroom culture.