Embodiments of the present invention concern methods of preventing malaria in a target population of humans comprising: uniformly administering a therapeutically effective amount of a live low dose malaria vaccine to each individual within the target population on each of two or three consecutive days. The methods include using Plasmodium infected mosquitoes to inject Plasmodium into individuals through mosquito bites.

The present invention is directed to a method of treating poultry hatchlings in a hatchling tray. The method comprises of providing a soft gel form capable of being dispensed through a spray nozzle, providing a spray dispensing apparatus, the apparatus being capable of delivering a predetermined volume of the gel as a plurality of small beadlets through a plurality of nozzles, placing the hatchling tray containing the hatchlings beneath the nozzles of the dispensing apparatus, dispensing the predetermined volume of the soft gel containing the therapeutic agent as small beadlets into the hatchling tray and allowing the hatchlings to consume the beadlets. The present invention is also directed to a dispensing apparatus for dispensing a therapeutic agent in a soft gel into a hatchling tray of poultry hatchlings.

The present invention is directed to a method of treating poultry hatchlings in a hatchling tray. The method comprises of providing a soft gel form capable of being dispensed through a spray nozzle, providing a spray dispensing apparatus, the apparatus being capable of delivering a predetermined volume of the gel as a plurality of small beadlets through a plurality of nozzles, placing the hatchling tray containing the hatchlings beneath the nozzles of the dispensing apparatus, dispensing the predetermined volume of the soft gel containing the therapeutic agent as small beadlets into the hatchling tray and allowing the hatchlings to consume the beadlets. The present invention is also directed to a dispensing apparatus for dispensing a therapeutic agent in a soft gel into a hatchling tray of poultry hatchlings.

Disclosed is a mutant strain of Sarcocystidae in which the two genes mic1 and mic3 are deleted containing two specific recombination sites of an enzyme allowing a specific recombination, in particular Cre-recombinase, the specific recombination sites being at the respective locus of each of the deleted genes, the specific recombination site of the enzyme allowing specific recombination, in particular of the Cre-recombinase, at the locus of the deleted mic1 gene being different from that of the deleted mic3 gene.

Disclosed is a mutant strain of Sarcocystidae in which the two genes mic1 and mic3 are deleted containing two specific recombination sites of an enzyme allowing a specific recombination, in particular Cre-recombinase, the specific recombination sites being at the respective locus of each of the deleted genes, the specific recombination site of the enzyme allowing specific recombination, in particular of the Cre-recombinase, at the locus of the deleted mic1 gene being different from that of the deleted mic3 gene.

Methods of preparing mammalian enteroids, and methods producing T. gondii oocysts in vitro and in vivo in heterologous systems, are provided.

Methods of preparing mammalian enteroids, and methods producing T. gondii oocysts in vitro and in vivo in heterologous systems, are provided.

The present disclosure provides systems and methods for delivering a gel to a surface. In one embodiment, the system may have a first vessel with a first low viscosity aqueous solution comprising a binder/crosslinking agent; and a second vessel with a second low viscosity aqueous solution comprising a gelling component. The separate first and second low viscosity aqueous solutions are sprayed onto a surface where the solutions mix forming a gel.

The present disclosure provides systems and methods for disrupting the outer membrane of an oocyst in solution and delivering the solution to an animal. The system includes a vessel containing unbroken oocysts in solution, an oocyst processing chamber, and a delivery outlet. The unbroken oocysts are moved from the vessel through the processing chamber and a portion of the oocyst membranes are disrupted releasing sporocysts, the resulting solution is moved from the processing chamber into the delivery outlet where the solution is delivered to an animal. Methods of vaccination, including vaccination against an Eimeria infection, are also provided.

The present disclosure provides systems and methods for disrupting the outer membrane of an oocyst in solution and delivering the solution to an animal. The system includes a vessel containing unbroken oocysts in solution, an oocyst processing chamber, and a delivery outlet. The unbroken oocysts are moved from the vessel through the processing chamber and a portion of the oocyst membranes are disrupted releasing sporocysts, the resulting solution is moved from the processing chamber into the delivery outlet where the solution is delivered to an animal. Methods of vaccination, including vaccination against an Eimeria infection, are also provided.