The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

A plurality of apparatus herein includes a locomotion device, a storage space with straps configured to be carried on a user's back, a trolley, or a wheelbarrow that each have a reflector body extending therefrom to which is mounted a UV-C light source operatively positioned relative to a reflector and operatively temperature controlled by an on-board temperature control unit to form a panel. The UV-C light source is operatively controlled to administer a dose of UV-C light based on a duration of exposure of at most two seconds when the panel has an optical power density between 300 W/m.sup.2 and 3000 W/m.sup.2 and a surface area of 0.01 m.sup.2 to 5 m.sup.2. The dose is in a range of 100 J/m.sup.2 to 1600 J/m.sup.2 as the locomotion device moves at an operating speed in a range of 1 km/hr to 10 km/hour.

A plurality of apparatus herein includes a locomotion device, a storage space with straps configured to be carried on a user's back, a trolley, or a wheelbarrow that each have a reflector body extending therefrom to which is mounted a UV-C light source operatively positioned relative to a reflector and operatively temperature controlled by an on-board temperature control unit to form a panel. The UV-C light source is operatively controlled to administer a dose of UV-C light based on a duration of exposure of at most two seconds when the panel has an optical power density between 300 W/m.sup.2 and 3000 W/m.sup.2 and a surface area of 0.01 m.sup.2 to 5 m.sup.2. The dose is in a range of 100 J/m.sup.2 to 1600 J/m.sup.2 as the locomotion device moves at an operating speed in a range of 1 km/hr to 10 km/hour.

The present disclosure provides a supplemental lighting method for vertical multi-layer cultivation of wrapper tobacco leaves, including the following steps: providing multi-layer vertical facilities, facilities including at least one cultivation rack having a plurality of layers; and providing supplemental lighting systems to each rack layer. For each rack layer the light intensity and light quality provided by the corresponding supplemental lighting system are set respectively according to a determined growth phase of the tobacco leaves during the cultivation process. The process includes cultivation of tobacco leaves through growth phases comprising a clumping period, a vigorous growth period, and a maturity period. During the clumping period, the light intensity provided comprises a first light intensity setting. During the vigorous growth period, the light intensity provided comprises a third light intensity setting. During the maturity period, the light intensity provided comprises a second light intensity setting.

The present disclosure provides a supplemental lighting method for vertical multi-layer cultivation of wrapper tobacco leaves, including the following steps: providing multi-layer vertical facilities, facilities including at least one cultivation rack having a plurality of layers; and providing supplemental lighting systems to each rack layer. For each rack layer the light intensity and light quality provided by the corresponding supplemental lighting system are set respectively according to a determined growth phase of the tobacco leaves during the cultivation process. The process includes cultivation of tobacco leaves through growth phases comprising a clumping period, a vigorous growth period, and a maturity period. During the clumping period, the light intensity provided comprises a first light intensity setting. During the vigorous growth period, the light intensity provided comprises a third light intensity setting. During the maturity period, the light intensity provided comprises a second light intensity setting.

The invention discloses gene sequences of a plant EPSPS mutant and its application in plants. As compared to the wild type, the present disclosure describes a plant EPSP synthase (EPSPS) polypeptide which comprises amino acid V mutating to amino acid A corresponding to the location at V403 or the analogous location at V403, wherein the amino acid position corresponds to the amino acid position set forth in SEQ ID NO:1.

The invention discloses an application method of medium and trace nutrient elements for wrapper hydroponic system. The application method includes the following steps: providing a nutrient solution cultivation system as well as medium and trace element nutrient solutions for facility-based cultivation of wrapper; the medium and trace element nutrient solutions are applied in the cultivation of wrapper; wherein the medium and trace element nutrient solutions contain modulated components, 65-420 ug/L boric acid and 230-1600 ug/L manganese salt, the modulated components contain at least two of 310-690 mg/L calcium salt, 400-1300 mg/L magnesium salt and 40-250 mg/L ferric salt. The present invention can obviously improve yield, appearance quality and leaf tensile force of the wrapper by applying the nutrient solution with specific components in different stage of the wrapper cultivation, respectively, thereby greatly improving the industrial availability of the wrapper raw materials.

The invention discloses an application method of medium and trace nutrient elements for wrapper hydroponic system. The application method includes the following steps: providing a nutrient solution cultivation system as well as medium and trace element nutrient solutions for facility-based cultivation of wrapper; the medium and trace element nutrient solutions are applied in the cultivation of wrapper; wherein the medium and trace element nutrient solutions contain modulated components, 65-420 ug/L boric acid and 230-1600 ug/L manganese salt, the modulated components contain at least two of 310-690 mg/L calcium salt, 400-1300 mg/L magnesium salt and 40-250 mg/L ferric salt. The present invention can obviously improve yield, appearance quality and leaf tensile force of the wrapper by applying the nutrient solution with specific components in different stage of the wrapper cultivation, respectively, thereby greatly improving the industrial availability of the wrapper raw materials.