Compositions and methods for producing reconstituted human skin and/or hair follicles in situ are provided. The method for producing the skin is unique in that tissue culture expanded cells including multipotent cells such as neonatal cells as well as cultured epidermal and dermal cells are immediately placed on a substrate such as a membrane and then the membrane with adherent cells is placed on a skin wound. Examples demonstrate formation of hair follicles in situ.

Cosmetic methods providing a significant improvement over existing hair transplantation methods by providing greater hair density per hair implant, which is achieved through the process of an enhanced hair multiplication in vivo (e.g. in the scalp of a subject). The cosmetic methods also provide improved aesthetic results, particularly, more realistic and natural looking results, relative to the results obtained by traditional methods. The cosmetic methods are particularly suitable for hair transplantation in recipient areas of a subject experiencing baldness or lack of hair, optionally due to Androgenic alopecia, burn injuries, cancer chemotherapy, or other genetic or environmental factors or scarring.

Provided herein are methods which alter the mechanical and biological properties of polymeric materials. Also provided are compositions comprising the polymeric materials having said properties.

The present invention provides a composition for stimulating hair growth in a mammal comprising a modified osteopontin polypeptide in which an RGD domain is inactivated; and a pharmaceutically acceptable and/or cosmetically acceptable excipient, carrier or diluent. The invention further provides methods of stimulating hair growth in a mammal.

Methods for promoting skin regeneration, promoting hair follicle regeneration, and reducing scarring by topically administering polysaccharide-based hydrogel compositions to injured skin are presented.

Compositions and methods involving microspheres composed of mesenchymal cells such as dermal papilla cells (DPCs) and extracellular matrix, epithelial cell such as keratinocyte are disclosed. It has been discovered that by balancing the proportion of DPCs and extracellular matrix in the DPC-matrix mixture, and by incubating a particular range of small volume of the DPCmatrix mixture, DPC-matrix microspheres can be formed that have useful properties. Most significantly, the resulting DPC-matrix microspheres are particularly suited to be used to produce bioengineered hair follicles that have features of native hair follicles.

The inventions provide methods of manufacturing peroxide cross-linked and high temperature melted polymeric material, for example ultra-high molecular weight polyethylene (UHMWPE) containing vitamin E, total joint implants with high wear resistance, high oxidation resistance, and very low concentration of residual by-products, as well as products made thereby.

The present invention provides a method for manufacturing a regular and high-density regenerated hair follicle primordium aggregation similar to the hair follicle tissue of a mammal in a simple manner. A regenerated hair follicle primordium aggregation manufacturing method of the present invention includes a step of forming hair follicle primordia by inoculating a microwell plate, which includes regularly arranged microwell portions, with mesenchymal cells and epithelial cells and culturing a mixture of the cells while supplying oxygen thereto.

Compositions containing purified collagen biomaterial derived from tissues, for example, insoluble amnion, soluble amnion, soluble chorion of the human placenta, are provided. The collagen compositions can be used to promote wound healing, promote tissue regeneration, prevent or reduce scarring, reduce local inflammation, minimize tissue rejection, promote graft integration. Methods for using the collagen composition as a biomaterial implant for dermal filling, skin grafting, and hair transplantation are also provided.

Provided is a method for regenerating hair follicles by transplanting a composition containing CD36-expressing dermal sheath cells (DSc) to a human. The composition may be prepared by sorting CD36-expressing dermal sheath cells from a hair follicle tissue by use of a cell-sorting technique along with an antibody to CD36. The composition may also contain dermal papilla cells (DPc).