Disclosed herein are methods of manufacturing injectable microgel scaffolds, including methods of producing, purifying and concentrating microgel particles therein. The microgel scaffolds of the present disclosure are useful for a wide range of applications, such as stabilizing an implanted medical device in an implant site in a subject. The microgel scaffolds are fluidic during application and annealed or crosslinked after application to the implant site in the subject. The microgel scaffolds may contain various therapeutic agents, including antibiotics and analgesics, throughout the gel.

Methods and materials for the cryopreservation of cellularised scaffolds used for therapeutic or pharmacological testing purposes that provide a cultured scaffold on which cells have been seeded, equilibrate the cellularised scaffold with a cryopreservative composition comprising culture medium and between 5 and 30% of a cryoprotectant such as DMSO, freeze the equilibrated cellularised scaffold by reducing the temperature continuously by about 1 C./minute to about 80 C., and store the frozen cellularised scaffold at a temperature of between 135 C. and 198 C.

The present disclosure relates to the field of biomaterials, tissue engineering and regenerative medicine. Particularly, the present disclosure relates to biomaterial composite and preparation of various scaffold formats using the composite. The disclosure provides for preparation of biomaterial composite comprising silk fibroin and melanin, preparation of scaffolds with the biomaterial composite and its applications in tissue engineering, electrotherapy applications and regenerative medicine. In some embodiments, the silk fibroin is a peptide modified silk fibroin. The biomaterial composite of the present disclosure exhibits various advantages including but not limiting to enhanced antioxidant property, superior electroactive properties, improved myogenic cell differentiation, better cell growth and regeneration, and enhanced cell adhesion property.

The present invention relates to an implantable device, in particular for wall repair comprising a reinforcing textile implant having first and second surfaces, a bioadhesive coating to coat said first surface at least in part, said coating comprising at least one ionic, cross-linked bioadhesive polymer selected from among the following polymers: an acrylic acid polymer, methacrylic acid polymer, itaconic acid polymer, maleic acid polymer or maleic anhydride polymer having an adhesive function that can be activated in an aqueous medium.

Provided herein are compositions and methods for treating a subject with damaged tissue, such as an injury associated with a tissue to tissue (e.g., a connective tissue-to-connective tissue or tissue to bone) interface. One aspect provides an adhesive film or adhesive layer, optionally comprising a biomaterial, tissue growth factors, including CTGF/CCN2, or cells.

Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition may also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function.

Disclosed herein are muscle implants and methods of making muscle implants comprising one or more decellularized muscle matrices. The muscle matrices can be provided in a particulate form suitable for injection or implantation.

The present invention refers to a method for the synthesis of a biodegradable calcium release material that shows controlled ion release properties for tissue engineering, biomaterials containing the calcium particles as well as the calcium particles obtainable therefrom. By varying the treatment temperature of the described method, the calcium material shows different calcium release profiles. Contrary to a specific chemical composition such as CaCO.sub.3 which is associated to a specific calcium release profile, the present invention allows a manifold of compositions, with a manifold of calcium release profiles, all starting from a single specific chemical composition calcium precursor. Therefore, the invention also relates to the use of the controllable release, calcium material in tissue regeneration such as wound healing processes.

The present invention relates to a biomaterial comprising a cartilage graft scaffold substantially free of viable cells, wherein the cartilage graft scaffold exhibits a plural of notches in form of lamellae or grids.

The present disclosure provides a method for preparing a decellularized muscle scaffold, decellularized muscle scaffolds produced therefrom, and uses thereof to treat a subject with damaged or lost muscle.