Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.

Described herein are compositions comprising decellularized extracellular matrix derived from skeletal muscle or other suitable tissue, and therapeutic uses thereof. Methods for treating, repairing or regenerating defective, diseased, damage, ischemic, ulcer cells, tissues or organs in a subject preferably a human, with diseases associated with muscular degeneration, using a decellularized extracellular matrix of the invention are provided. Methods of preparing culture surfaces and culturing cells with absorbed decellularized extracellular matrix are provided.

Described herein are compositions comprising decellularized extracellular matrix derived from skeletal muscle or other suitable tissue, and therapeutic uses thereof. Methods for treating, repairing or regenerating defective, diseased, damage, ischemic, ulcer cells, tissues or organs in a subject preferably a human, with diseases associated with muscular degeneration, using a decellularized extracellular matrix of the invention are provided. Methods of preparing culture surfaces and culturing cells with absorbed decellularized extracellular matrix are provided.

The invention relates to a method for preparing a polymer scaffold that comprises the steps of providing a piece of a fabric of filaments of a first biodegradable or biocompatible polymer, applying a coating of a second polymer to said arrangement of filaments, and stretching the piece along its axis of longitudinal extension, thereby obtaining an aligned microfibrillar scaffold. The invention further relates to a method for providing an artificial tissue, and to a microfibrillar scaffold of aligned filaments obtained by the method of the invention.

The present invention relates to a biomaterial fabrication process for the manufacture of a collagen based fabric for an aligned collagen fiber network.

The present invention provides a prosthetic device including a pad-shaped spacer (100) including expandable layers (30) having a structure that expands in response to the introduction of moisture because the expandable layers (30) are made of a hydrogel material, so that in a surgery or treatment process, water is absorbed into the expandable layers (30) and fill a space inside a muscle or joint (J) tissue in a state in which the spacer (100) is implanted inside the space inside the muscle or joint (J) tissue until a new tissue grows inside the muscle or joint (J) tissue for a predetermined period of time, and the expandable layers (30) degrade gradually at a rate corresponding to a rate at which the tissue grows, with the result that the muscle or joint (J) tissue can be used during a recovery period.

Tissue compositions and methods of preparation thereof are provided. The tissue compositions can be used to treat or regenerate muscle tissue. The compositions can be configured to provide increased strength compared to other muscle matrices.

The present invention provides methods of regenerating muscle tissue and methods of treating volumetric muscle loss comprising administering decellularized fetal matrix scaffold. Also provided are methods for treating soft tissue injury and improving reconstructive surgery.

A method for treating damaged or diseased mammalian tissue that includes the steps of (i) providing a sheet structure formed from acellular dermal mammalian tissue that includes extracellular matrix (ECM) and a supplemental bioactive component, such as a nucleic acid, and/or a cell, and (ii) delivering the sheet structure to the damaged or diseased mammalian tissue, wherein the sheet structure induces angiogenesis and, thereby, regeneration of new mammalian tissue.

The present invention relates to the field of regenerative medicine, and more particularly to the improvement of the in vivo engraftment potential of biological material to be administered to a subject in need thereof.