Disclosed herein are compositions and methods for labeling cells using click chemistry reagents. The compositions and methods disclosed herein provide a specific and efficient means of localizing desired agents to a variety of cell types in vivo and in vitro.

An ultrasound treatment head and an ultrasound imaging and treatment method using the same are disclosed. The ultrasound treatment head according to an embodiment comprises: a structure including a treatment transducer and an imaging probe positioned at the center of the treatment transducer, the treatment transducer and the imaging probe being physically aligned; a first mechanism for steering the mechanical movement of the entire structure; and a second mechanism for steering the mechanical movement of either the imaging probe or the treatment transducer alone in the structure.

A device for inducing production of tears may include a body extending from a proximal end to a distal end. The body may be configured for insertion through a puncta of a subject. The device also may include a stimulus delivery mechanism positioned between the proximal end and the distal end and an induction coil operably coupled to the stimulus delivery mechanism. Further, the device may include an external controller wirelessly coupled to the induction coil for inductively transferring energy to the induction coil.

A histotripsy therapy system configured for the treatment of tissue is provided, which may include any number of features. Provided herein are systems and methods that provide efficacious non-invasive and minimally invasive therapeutic, diagnostic and research procedures. Treatment planning systems are also provided for preparing and implementing a digital treatment plan. The treatment planning can include dividing a target tissue volume into a plurality of treatment locations, and controlling the speed, and pattern of movement between these tissue locations.

A method of diagnosis and treatment of tumors using High Intensity Focused Ultrasound is provided. The method of diagnosing the presence of a tumor in a patient comprises the steps of subjecting a tumor to high intensity focused ultrasound (HIFU) to cause the tumor cells to release cellular material and evaluating the cellular material for a tumor marker. The method of treating a tumor in a patient can also comprise the step of subjecting a tumor to high intensity focused ultrasound (HIFU) to provoke an immune response.

The invention includes: a new composition of matter (a composite comprising a naturally occurring in vivo cornea in an in situ eye together with at least one volume of vitrified non-naturally occurring corneal stromal tissue formed within the naturally occurring corneal stromal tissue) wherein the vitrified tissue is modified in structure and properties from its naturally occurring condition into a non-naturally occurring glass-like condition with modifications including but not limited to increased eletastic modulus; methods for producing and using the new composition of matter for modifying cortical structure and properties, including but not limited to corneal optical aberrations; wound closure adhesion and transplant adhesion; and a photovitrification system for producing the new composition of matter comprising at least one photon source with controllable treatment parameters. A reverse template can be added to corneal vitrification systems to increase vitrification and modifications of structure and properties.

The invention is directed to a method of non-invasively treating a varicose vein (2), for example the Great saphenous vein (GSV), wherein the vein or the vein system to be treated is divided into sections (6a, 6b, 6c, 6d) with a length (11) separated by a spacing (s1). The sections can be pre-defined before the treatment, for example to target a junction (4) to a tributary (3), or with respect to the sapheno-femoral junction (8), and can vary in length and location, for example to avoid a sensitive structure (7) such as nerves.

The present invention relates to a sonosensitizer composition containing titanium dioxide nanoparticles as an active ingredient, a composition for preventing or treating cancer containing the same, and a method of preparing the same. The sonosensitizer of the present invention can solve the problem of the conventional photosensitizer, which can be used only when light can reach the target area, and increase the accumulation level of the sonosensitizer of the present invention to a maximum level within a short time, thereby enabling reduction of the time required for treatment and, due to its human-friendly stability, effective use in cancer treatment.

The invention includes: a new composition of matter (a composite comprising a naturally occurring in vivo cornea in an in situ eye together with at least one volume of vitrified non-naturally occurring corneal stromal tissue formed within the naturally occurring corneal stromal tissue) wherein the vitrified tissue is modified in structure and properties from its naturally occurring condition into a non-naturally occurring glass-like condition with modifications including but not limited to increased elastic modulus; methods for producing and using the new composition of matter for modifying corneal structure and properties, including but not limited to corneal optical aberrations; wound closure adhesion and transplant adhesion; and a photovitrification system for producing the new composition of matter comprising at least one photon source with controllable treatment parameters. A reverse template can be added to corneal vitrification systems to increase vitrification and modifications of structure and properties.

A method for reducing foreign body response in a subject caused by an electrode implanted in a subject's neural tissue at a target site provides positioning a device having a body, ultrasonic transducer, and acoustic coupling medium in contact with the neural tissue proximate to the target site. The method continues with activating a transducer, generating acoustic vibrations, transmitting the acoustic vibrations to the neural tissue at the target site, and applying the acoustic vibrations to the neural tissue at the target site sufficient to reduce the foreign body response in the neural tissue at the target site. The acoustic vibrations are preferably in the ultrasonic range and are pulsed for a duration of 5-200 milliseconds. A treatment protocol may consist of repeat sessions in which the transducer is activated and the acoustic vibrations are applied for 1-15 minutes, then turned off for 1-15 minutes, and repeated 2-10 times.