Pepper plants exhibiting resistance to root knot nematode species are provided, together with methods of producing, identifying, or selecting plants or germplasm with a root knot nematode resistance phenotype. Such plants include pepper plants comprising introgressed genomic regions conferring pest resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed pest resistance alleles, are further provided.

The reported method identifies the likelihood that a heritable sequence in a plant is to be passed onto subsequent generations. One or more genome edits are introduced into a plant cell and the gnomic DNA of the resulting plant is analyzed to determine the frequency of the one or more genome edits in the sample. Edits that are present in a quantity above a reference cutoff are considered to have a high likelihood of being heritable and capable of being passed on to subsequent generations. Edits that are present in low quantities are considered to have a low likelihood of being passed on. Plants containing desirable genome edits that are likely to be heritable are then used for cultivation and propagation.

The reported method identifies the likelihood that a heritable sequence in a plant is to be passed onto subsequent generations. One or more genome edits are introduced into a plant cell and the gnomic DNA of the resulting plant is analyzed to determine the frequency of the one or more genome edits in the sample. Edits that are present in a quantity above a reference cutoff are considered to have a high likelihood of being heritable and capable of being passed on to subsequent generations. Edits that are present in low quantities are considered to have a low likelihood of being passed on. Plants containing desirable genome edits that are likely to be heritable are then used for cultivation and propagation.

Provided are a method for improving rice yield by jointly knocking out ABA receptor PYL family genes and a use thereof.

An Agrobacterium-mediated genetic transformation method for sea barleygrass is provided. The method includes: S1, selecting immature embryo materials of sea barleygrass with immature embryos each having a length in a range of 0.5-1.0, sterilizing them with alcohol and sodium hypochlorite to obtain sterilized seeds; S2, separating the immature embryos, crosscutting the immature embryos, and inducing callus generation and proliferation; S3, adjusting pre-culture time and Agrobacterium infection time of calli based on the callus generation and Agrobacterium growth to thereby prevent excessive Agrobacterium liquid; and S4, performing adventitious bud induction culture and rooting induction culture under a shielding-formed low-light environment to obtain tissue culture plantlets. It relates to a tissue culture method for immature embryos of sea barleygrass with high green spot differentiation and plantlet formation rates, which is not limited by materials. A transformation and regeneration system has high genetic transformation and mutation efficiency.

An Agrobacterium-mediated genetic transformation method for sea barleygrass is provided. The method includes: S1, selecting immature embryo materials of sea barleygrass with immature embryos each having a length in a range of 0.5-1.0, sterilizing them with alcohol and sodium hypochlorite to obtain sterilized seeds; S2, separating the immature embryos, crosscutting the immature embryos, and inducing callus generation and proliferation; S3, adjusting pre-culture time and Agrobacterium infection time of calli based on the callus generation and Agrobacterium growth to thereby prevent excessive Agrobacterium liquid; and S4, performing adventitious bud induction culture and rooting induction culture under a shielding-formed low-light environment to obtain tissue culture plantlets. It relates to a tissue culture method for immature embryos of sea barleygrass with high green spot differentiation and plantlet formation rates, which is not limited by materials. A transformation and regeneration system has high genetic transformation and mutation efficiency.

This invention describes a new, high-efficiency method of selecting and advancing pollen donator strains in a breeding or product advancement program, wherein the pollen donator strains are specifically selected to maximize product attributes. Embodiments of this invention relate to the use of a mix of pollen including a cross-pollination source and self-pollen, allowing for single-plant performance comparisons. The comparisons of products from the single plant or less experimental unit allow for the selection of those pollen donator strains that maximize desirable results.

Isolated DNA of the Wtk1 gene or a functional equivalent capable of conferring resistance to stripe rust, is provided, as well as artificial vectors comprising same, proteins encoded by same and nucleic acid molecules for detecting same. Transgenic plants, as well as cells, seeds, and tissue therefrom which express the Wtk1 gene or a functional equivalent thereof are also provided.

Methods, compositions, kits, and computer program code are provided for predicting somaclonal abnormality (e.g., a Mantled phenotype) in a plant and or sorting plants based on the predicted presence or absence of somaclonal abnormality.

Methods, compositions, kits, and computer program code are provided for predicting somaclonal abnormality (e.g., a Mantled phenotype) in a plant and or sorting plants based on the predicted presence or absence of somaclonal abnormality.