Methods of treating tumors by administering compounds to a patient are provided. Compounds such as pro drugs, e.g., 5-aminolevulinic acid (5-ALA), may be administered to the patient orally, by injection, intravenously, or topically, which then accumulate preferentially as compounds such as protoporphyrin IX (PpIX) in tumor cells. After such accumulation, compounds such as PpIX are then activated in various aspects to treat tumors cells, thereby treating cancer. Cancers such as glioblastoma may be treated.

The system and process of therapeutic and effective cavitation by using ultrasound to collapse gas vesicles as well as cavitate the bubbles produced from the collapsed gas vesicles. Therapeutic effect includes, but is not limited to lysing cells by cavitation. The cells expressing the gas vesicles can optionally be used as delivery cells to preform tasks such as transporting the gas vesicles into deep tissue areas, releasing compounds at the cavitation site, and more. The gas vesicles can optionally be modified to facilitate getting the bubbles near the cavitation targets by functionalizing the gas vesicles.

A method for mid-intensity non-ablative acoustic treatment of injured tissue is disclosed. The method involves continuously moving an ultrasound probe across an extracorporeal skin surface while emitting non-ablative therapeutic ultrasound into the injured tissue in a non-ablative therapeutic ultrasound beam profile. The method terminates energy delivery if movement speed is below a speed threshold. The non-ablative therapeutic ultrasound beam profile provides substantially uniform heating throughout a treatment volume. The heating is non-ablative and triggers a healing response in the injured tissue.

Methods for treating skin and subcutaneous tissue with energy such as ultrasound energy are disclosed. In various embodiments, ultrasound energy is applied at a region of interest to affect tissue by cutting, ablating, micro-ablating, coagulating, or otherwise affecting the subcutaneous tissue to conduct numerous procedures that are traditionally done invasively in a non-invasive manner. Methods of lifting sagging tissue are described.

The subject matter of the present disclosure generally relates to techniques for following a treatment protocol having one or more treatment parameters to cause a targeted physiological outcome at a distal site, assessing an expression level of a gene in a region of interest after completing the treatment protocol, and modifying the one or more treatment parameters based on the expression level of the gene. The treatment protocol may include one or more ultrasound energy treatments to the region of interest.

Systems and methods for a soft tissue treatment of a patient are provided herein. The device for a soft tissue treatment may include an applicator having at least one electrode, a fastening mechanism to fix the applicator to a body part of the patient, and a control unit having a microprocessor to control the at least one electrode. The at least one electrode may provide radiofrequency energy and pulsed electric current. The radiofrequency energy may cause a heating of a soft tissue. The electric current may cause contraction of a muscle within the body part. The body part may be a face.

A real-time acoustic simulation method based on artificial intelligence according to an embodiment of the present disclosure includes acquiring medical image data of a target area to be treated; determining ultrasound parameters related to the output of an ultrasonic transducer based on the medical image data; inputting the ultrasound parameters to a numerical model to generate a numerical model based acoustic simulation image for a specific position of the ultrasonic transducer; training an artificial intelligence model using the medical image data and the numerical model based acoustic simulation image; generating an artificial intelligence model based acoustic simulation image for an arbitrary position of the ultrasonic transducer using the trained artificial intelligence model; and outputting a real-time acoustic simulation image with a position change of the ultrasonic transducer.

Embodiments of a dermatological cosmetic treatment and/or imaging system and method adapted for dithering ultrasound beams from a transducer to alter placement and position of one or multiple cosmetic treatment zones in tissue, simultaneous multi-focus therapy using multi-channel signal mixing, and/or dithering ultrasound beams from a transducer to alter placement and position of one or multiple cosmetic treatment zones in tissue, configured for using imaging for improved ultrasound therapy efficacy, and/or adapted for imaging with multiple focal zone sequencing and triggering for mechanically translated and/or steered ultrasound transducers are provided herein. The system can include a hand wand, a removable transducer module, and a control module. In some embodiments, the cosmetic treatment system may be used in various cosmetic procedures.

A therapeutic ultrasound system transmits a staggered or interleaved pattern of therapy beams for use in sonothrombolysis and other Vascular Acoustic Resonators (VAR) mediated therapy. The inventive technique minimizes VAR, e.g. microbubble, destruction due to adjacent beams, ensures uniform sonication of the targeted region by filling in the spaces between the beams in subsequent passes, and further provides a means for bubble replenishment to maximize the clot lysis from ultrasound. The technique is also applicable to diagnostic ultrasound, VAR mediated drug delivery and blood brain barrier opening.

Embodiments of the inventive concept provide an ultrasonic medical instrument moving horizontally by adjusting a depth wise location of an ultrasonic wave generating unit while not influencing an internal volume of a cartridge and variously setting focusing depths of ultrasonic waves in skin.