A spectrum for promoting growth of cannabis plants. The spectrum comprises adding an irradiation of red light with peak wavelength at 655-690 nm into an indoor growing environment of cannabis to improve the levels of THC and CBD, the cannabinoid substances in cannabis. A ratio of photon number of the red light to the photon number of the entire light source is in a range from 40% to 70%. While maintaining the light intensity and other growth conditions, the yields and levels of THC and CBD, the cannabinoid substances in cannabis, can be increased by up to 32.48%.

The present invention provides a method for improving a one-time seedling rate of microspore embryoids of Brassica campestris ssp. Chinensis Makino, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl.sub.2; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4° C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25° C. and 14 h of illumination by blue-red compound light/day until seedlings.

This disclosure relates to methods and compositions with enhanced levels of one or more tyramine containing hydroxycinnamic acid amides. Also disclosed herein are methods for producing a consumable product with enhanced levels of a tyramine containing hydroxycinnamic acid amide. Some embodiments relate to a composition enriched with a tyramine containing hydroxycinnamic acid.

A method for promoting the accumulation of secondary metabolites of cannabis is disclosed. The method comprises the step of adding an irradiation of green-yellow light, which has a peak wavelength at 505-590 nm, into the indoor growing environment of cannabis to improve the level of cannabidiol (CBD), secondary metabolites in cannabis. While maintaining the light intensity and other growth conditions, the yield and/or level of CBD, secondary metabolites in cannabis, can be increased by up to 11.04%.

A method for promoting the accumulation of secondary metabolites of cannabis is disclosed. The method comprises the step of adding an irradiation of green-yellow light, which has a peak wavelength at 505-590 nm, into the indoor growing environment of cannabis to improve the level of cannabidiol (CBD), secondary metabolites in cannabis. While maintaining the light intensity and other growth conditions, the yield and/or level of CBD, secondary metabolites in cannabis, can be increased by up to 11.04%.

A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimised depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimised depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

The present invention relates to a method for preparation of soybean leaves having a high content of an isoflavone derivative in a dark condition and soybean leaves having a high content of an isoflavone derivative prepared thereby. Specifically, treatment of a soybean plant 20 days to 60 days after seeding with a predetermined concentration of ethylene in a dark condition was found to accumulate higher concentrations of isoflavone derivatives in soybean leaves than treatment with ethephon in a light condition, which requires a high level of energy. Therefore, when used, the method of the present invention can economically and quickly prepare soybean leaves having a very high content of isoflavones, and the soybean leaves having a high content of isoflavone derivatives, an extract of the soybean leaves, and a fraction of the extract can be advantageously used as a food and medicine material against diseases caused by estrogen unbalance and deficient antioxidant activity.

The present invention relates to a method for preparation of soybean leaves having a high content of an isoflavone derivative in a dark condition and soybean leaves having a high content of an isoflavone derivative prepared thereby. Specifically, treatment of a soybean plant 20 days to 60 days after seeding with a predetermined concentration of ethylene in a dark condition was found to accumulate higher concentrations of isoflavone derivatives in soybean leaves than treatment with ethephon in a light condition, which requires a high level of energy. Therefore, when used, the method of the present invention can economically and quickly prepare soybean leaves having a very high content of isoflavones, and the soybean leaves having a high content of isoflavone derivatives, an extract of the soybean leaves, and a fraction of the extract can be advantageously used as a food and medicine material against diseases caused by estrogen unbalance and deficient antioxidant activity.

Embodiments described herein provide systems for inducing a desired response in an organism by controlling the duty cycle, wavelength band and frequency of photon bursts to an organism, through the photon modulation of one or more photon pulse trains in conjunction with one or more different photon pulse trains to the organism and duty cycle, where the photon modulation and duty cycle is based upon the specific needs of the organism. Devices for inducing a desired response in an organism such as growth, destruction or repair through the photon modulation of one or more photon pulse trains in conjunction with one or more different photon pulse trains to the organism are also provided. Further provided are methods for the optimization of organism growth, destruction or repair through the use of high frequency modulation of photons of individual color spectrums.