Disclosed herein are methods for the production of Cannabis meristem explants from dry seeds. Also described are methods of transforming and gene editing using the Cannabis meristem explants disclosed herein.

The invention relates to food industry and medicine and describes the method of producing transformed plant cells, containing recombinant human alkaline phosphatase, and their use for maintaining the homeostasis of gastrointestinal tract as an agent, regulating gastrointestinal tract microflora and the immune system.

Systems and methods for editing plant genomes include systems and methods for transforming plants generally, and Brassica plants particularly, with genome editing systems including CRISPR-Cas. Systems and methods for editing plant genomes include systems and methods for transforming Brassica microspores with genome editing systems and culturing the plant microspores that increase genome editing frequency.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

The present invention is a single blade clone cutter. The single blade clone cutter comprises a u-shaped housing with a single removable blade. The blade is locked into the u-shaped housing to secure the blade from moving during the process of cutting. It is important to have a single blade that cuts onto a flat surface so there is no damage being done to the fibers at the end of a cutting.

A bioreactor comprising: an upper container with transparent walls for the material being propagated, the container being provided with gas exchange diffuser to the external environment, humidifier, and artificial illumination; a lower container with transparent walls, having an aluminum tray and a point of water entry and/or nutrient medium, located in the bottom of the container; points of injection/removal of air/oxygen/carbon dioxide located in the lid; point of additional injection of carbon dioxide, close to the bottom of the upper container; hermetic connection device between the upper and lower containers for supply/drainage of the nutrient medium; a screen support for the material to be propagated; locking devices to hermetically close the upper container; and the lower container; and pneumatic drivers of the liquid nutrient medium between the upper container and the lower container.

A pick-and-place system for plant embryos includes a tray for receiving a suspension of plant embryos. The tray is movable to repeatedly reposition the plant embryos. An identification system is proximate the tray for identifying a target plant embryo amongst the plant embryos in the suspension. The identification system is configured to identify the target plant embryo while the tray is moving. A robotic arm is proximate the tray and is in communication with the identification system. The robotic arm is actuatable to pick the target plant embryo out of the suspension while the tray is moving, and deposit the target plant embryo at a target location.

Disclosed herein are methods for sterile extraction, drying, storage, and transformation of immature and mature maize and monocot embryos. The present disclosure also describes a dried, storable, freezable value added maize and monocot explants.

Disclosed herein are media, kits, systems and methods for achieving micropropagation of a T-R date palm on a commercially relevant scale. Compositions and methods for each stage of micropropagation, including initiation, elongation, and rooting are disclosed in the present application. Also disclosed are conditions for harvesting and sterilizing explant tissue for micropropagation.

Disclosed herein are methods of producing substantially pathogen-free plants of the genus Cannabis and pathogen-free plants and clones produced therefrom. One embodiment of the method comprises pretreating a progenitor plant of the genus Cannabis, surface sterilizing a shoot segment of the pretreated plant with a bleach solution, excising a meristematic tip of the shoot segment, and transferring the meristematic tip into a culturing plate comprising a supplemented Murashige and Skoog culture medium for further culturing. The supplemented Murashige and Skoog culture medium can comprise benzyladenine, naphthaleneacetic acid, gibberellic acid, or a combination thereof.