The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to a watermelon plant showing resistance against potyviruses, wherein the watermelon plant may comprise one or more of resistance conferring alleles of QTL1, QTL2 and QTL3 and wherein the said QTLs are as present in or obtainable from a watermelon plant, representative seed of which was deposited under deposit accession number NCIMB 42537, 42536, 42535 respectively.

This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via co-delivery of one or more chemicals, such as protein deacetylase inhibitors, phytohormones and/or regeneration boost genes, with genome engineering components.

Provided herein are systems, methods and kits for micropropagating disease-free cannabis plants, referred to as the Clean Stock® method. Also provided are Clean Stock® cannabis plants produced using such systems, methods and kits.

New lettuce varieties designated ‘Ezthana’ and ‘Eztron’ are described. ‘Ezthana’ and ‘Eztron’ are lettuce varieties exhibiting stability and uniformity.

A circular integrated ecological breeding and planting system including a plurality of units capable of growing vegetables, producing worms, breeding and raising chickens, and growing seaweed is provided to collectively construct a simplified simulated food. It not only unifies the lower and upper ecological food chains, but also minimizes pollution and undesirable waste. The system is particularly suitable for enclosed and intensive production within a single enclosed building to greatly reduce the production cost. This proposed eco-agricultural model has applications beyond its current configuration and can be applied to grow other plants, and to breed and raise other animals in the future.

A hormone-free culture method for a citrus shoot tip is provided, including: (1) selecting a citrus branch to make an explant; (2) washing with sterile water after disinfection; (3) inserting the explant into a Murashige and Skoog (MS) solid medium and culturing to produce a branch with new buds; (4) preparing a Murashge and Tucker (MT) liquid medium solution; (5) taking a shoot tip, and inoculating in a liquid medium for primary culture; (6) selecting a seed of a trifoliate orange or a citrange and peeling off a testa; washing with the sterile water after disinfection; (7) inoculating a resulting seed into a test tube containing the MS solid medium to obtain an etiolated seedling; (8) cutting a T-shaped incision and placing the shoot tip in a middle of the T-shaped incision to obtain a grafted seedling; and (9) conducting a secondary grafting.

A breeding method of a polyploid rice two-line restorer line includes determining hybrid parents for breeding the restorer line; breeding a restorer line of an indica sterile/japonica restorer type, or breeding a restorer line of a japonica sterile/indica restorer type, and carrying out backcrossing or composite hybridization after hybridization of the parents; selecting a single plant that meets the breeding goal, carrying out composite hybridization again and carrying out preliminary molecular marker detection; comparing, and selecting a single plant with good traits for continuous selfing until the line is basically stable; selecting a stable line with good traits and detection molecular markers; carrying out test-crossing on the preferred line as a male parent with different types of polyploid sterile lines; and selecting a good hybrid combination and a restorer line thereof.

The semi-dwarf, mid-season, medium grain rice variety MM17 is disclosed. The invention relates to the seeds and plants of the semi-dwarf, mid-season, medium grain rice variety MM17. The invention also relates to methods for producing a rice plant produced by crossing the semi-dwarf, mid-season, medium grain rice variety MM17 with itself or another variety.

The present invention is directed to a transgenic plant and a method for producing the same. In particular, the present invention is directed to a transgenic plant or a plant cell in which a nucleic acid molecule encoding an m.sup.6A demethylase is introduced, wherein said m.sup.6A demethylase has the following two domains: i) N-terminal domain (NTD) having the function of AlkB oxidation demethylase; and ii) C-terminal domain (CTD). The present invention is also directed to a method for producing said plant, comprising introducing a nucleic acid molecule encoding an m.sup.6A demethylase into a regenerable plant cell, and regenerating a transgenic plant from the regenerable plant cell.