Methods of propagating a Cannabis cutting are provided. A method of propagating a Cannabis cutting can include: providing a coherent growth substrate comprising man-made vitreous fibres bonded with a cured binder composition, the growth substrate having a density in the range of 60 kilograms per cubic meter (kg/m.sup.3) to 70 kg/m.sup.3; inserting the Cannabis cutting into the growth substrate at a location where the growth substrate does not have a seed hole; and providing a nutrient solution having an electrical conductivity value between 1.6 milli-Siemens per centimeter (mS/cm) and 2.4 mS/cm to the Cannabis cutting in the growth substrate.

Methods of propagating a Cannabis cutting are provided. A method of propagating a Cannabis cutting can include: providing a coherent growth substrate comprising man-made vitreous fibres bonded with a cured binder composition, the growth substrate having a density in the range of 60 kilograms per cubic meter (kg/m.sup.3) to 70 kg/m.sup.3; inserting the Cannabis cutting into the growth substrate at a location where the growth substrate does not have a seed hole; and providing a nutrient solution having an electrical conductivity value between 1.6 milli-Siemens per centimeter (mS/cm) and 2.4 mS/cm to the Cannabis cutting in the growth substrate.

The present invention relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid plants of the family Cucurbitaceae from isolated microspores, wherein said method comprises a) culturing isolated microspores to obtain embryos competent for plant regeneration, wherein the microspores have been isolated from plant material of a donor plant of the family Cucurbitaceae; and b) regenerating plants from the embryos; wherein step (a) comprises contacting the microspores with one or more inhibitor of histone deacetylase (HDACi) and one or more polyamine. The present invention also relates to a method for producing haploid, dihaploid, polyhaploid and/or doubled haploid embryos, to related kits and compositions, and to plants obtained according to the methods.

The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus formation and regeneration of plants from callus tissue using a histone deacetylase inhibitor.

The present invention relates to a method for Agrobacterium-mediated transformation and regeneration of Stevia plants. In particular, the method involves co-culturing leaf explants with Agrobacterium in a medium comprising acetosyringone and 2,4-dichlorophenoxyacetic acid in the dark, callus induction and shoot regeneration in a medium comprising 6-benzylaminopurine, 3-indoleacetic acid, a selective agent and an Agrobacterium eradicant in the dark, and root regeneration in a medium comprising 3-indoleacetic acid in a light/dark cycle. The present invention also relates to the overexpression of SrDXS1 and SrKAH in transgenic plants, resulting in the enhancement of steviol glycosides in the transgenic plants. The present invention further relates to the overexpression SrUGT76G1 in transgenic plants, resulting in higher Rebaudioside A (Reb A) to stevioside ratios in the transgenic plants.

A tissue culture method and a propagation method of Cathaya argyrophylla are described herein. An adventitious bud can be induced from an explant of the Cathaya argyrophylla using a suitable induction medium, with an induction rate reaching 69.22% to 73.33%. The induced adventitious bud is inoculated into a suitable proliferation medium, such that the adventitious bud can proliferate and strengthen seedlings, where the adventitious bud shows a proliferation rate reaching 46.23% to 55%. The adventitious bud after proliferation and seedling strengthening are inoculated into a suitable rooting medium to allow rooting culture, thereby obtaining a complete tissue culture seedling of the Cathaya argyrophylla. The tissue culture method is of great significance for protecting the Cathaya argyrophylla, increasing a plant quantity of the Cathaya argyrophylla, and expanding a population of the Cathaya argyrophylla to prevent extinction.

Novel cultivars of Stevia rebaudiana plant, with a novel genetic trait of self-compatibility, and the advantageous use of this genetic trait in Stevia crossing breeding for increasing steviol glycosides production, including food and beverage products and other consumables, are disclosed.

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

The present invention relates to the field of plant breeding and in particular to the generation of plants from cells and other tissues. More particularly, the invention provides methods and means for improving plant regeneration, especially from transformed or genetically modified plant cells.

The invention pertains to a method for regenerating a plant cell, preferably regenerating a shoot from a plant cell by altering the expression levels of at least WOX5 and a PLT protein, preferably WOX5 and PLT1. In addition the expression levels of further proteins can altered, such as WIND1, SHR, SCR, RBR, PLT4 and PLT5 to regenerate a shoot from a plant cell. Preferably, the expression levels are transiently altered. The invention further pertains to a nucleic acid construct suitable for transient protein expression and the use of the protein combinations for regenerating a shoot from a plant cell.