Provided herein are devices, systems, and methods for airborne rooting and/or callusing of cuttings. The device includes a container defining an interior that can be humidified to allow for development of calluses or roots.

The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

A breeding method of a polyploid rice photo-thermo-sensitive genetic male sterile line includes determining a diploid rice line with photo-thermo-sensitive genetic male sterility or PMeS characteristic as a parent; carrying out hybridization on a diploid photo-thermo-sensitive genetic male sterile line and a diploid PMeS gene line, carrying out doubling culture on a young ear of a hybrid plant into a hybrid tetraploid; back-crossing the hybrid tetraploid with a tetraploid photo-thermo-sensitive genetic male sterile line; selecting a tetraploid male sterile plants from the back-crossed progeny, self-crossing during a low-temperature and short-day fertile period, and then carrying out composite hybridization with another tetraploid rice line having PMeS gene; selecting tetraploid male sterile plants, and detecting the stability of tetraploid male sterile plants after multiple generations of continuous self-crossing; and determining the stable and consistent tetraploid rice sterile line as the polyploid rice photo-thermo-sensitive genetic male sterile line, named as PSXXX.

Disclosed herein are compositions and methods for effecting alterations at a defined location in the genome of a plant cell or plant protoplast. Further disclosed are methods using an RNA-guided nuclease to alter a target nucleotide sequence in a cell or protoplast of a plant; embodiments of such methods include treatments with chemical or physical reagents or treatment of the cell or protoplast with a specific thermal regime, such as a heat treatment.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

Disclosed herein are media, kits, systems and methods for achieving micropropagation of a T-R date palm on a commercially relevant scale. Compositions and methods for each stage of micropropagation, including initiation, elongation, and rooting are disclosed in the present application. Also disclosed are conditions for harvesting and sterilizing explant tissue for micropropagation.

Materials and methods for genome engineering through transient expression of a targeted nuclease are described herein. For example, the methods described herein can include introducing into a cell a messenger RNA (mRNA) that encodes a nuclease targeted to a selected sequence within the cell, where the stability of the mRNA is modified by the addition of untranslated regions (UTRs).

Disclosed herein are methods of producing substantially pathogen-free plants of the genus Cannabis and pathogen-free plants and clones produced therefrom. One embodiment of the method comprises pretreating a progenitor plant of the genus Cannabis, surface sterilizing a shoot segment of the pretreated plant with a bleach solution, excising a meristematic tip of the shoot segment, and transferring the meristematic tip into a culturing plate comprising a supplemented Murashige and Skoog culture medium for further culturing. The supplemented Murashige and Skoog culture medium can comprise benzyladenine, naphthaleneacetic acid, gibberellic acid, or a combination thereof.

The invention discloses a medium combination for a rapid propagation medium for blueberry stem segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stem segment tissue, the propagation rate of this method is rapid.