A biological product for the purification of water, soil, industrial wastewater from chemicals that are resistant to degradation, such as pesticides and oil, contains a composition of strains of bacteria Pseudomonas putida VKPM B-10997, Bacillus subtilis VKPM B-10999, and Rhodococcus erythropolis VKPM Ac-1882 in a weight ratio of (1-2);(1-2):1. The biological product can further contain sorbent, organic and mineral supplements, and stimulating agents. The biological product can be used for the decomposition of degradation-resistant pesticides and oil and has plant growth stimulating and fungicidal activity.

Certain exemplary embodiments provide methods for reducing a concentration of a contaminant associated with a medium, which can be any substance or material, such as soil, water, air, and/or fluid. In one exemplary method, the medium is treated with a ferric chelate and an oxidizing agent in amounts effective to oxidize at least a portion of the contaminant.

The present invention relates to the field of applied and environmental microorganism and agriculture. Disclosed are a thifensulfuron hydrolase gene tsmE and uses thereof. The thifensulfuron hydrolase gene tsmE has a nucleotide sequence of SEQ ID NO. 1, full length of 1194 bp, and G+C content of 51.09%, and encodes 398 amino acids with an amino acid sequence of SEQ ID NO. 2. The thifensulfuron hydrolase TsmE provided by the present invention can degrade completely 100 mg/L thifensulfuron within 1 hour into the herbicidally inactive product thiophenesulfonic acid; in addition, the TsmE also degrade completely 100 mg/L haloxyfop-R-methyl within 1 hour. Therefore, the thifensulfuron hydrolase gene tsmE is useful in construction of thifensulfuron-resistant transgenic crops and bioremediation of thifensulfuron or haloxyfop-R-methyl-contaminated environments.

The present invention is directed to a formulation and associated method for neutralizing one or more toxic chemical and/or materials including toxic industrial chemicals and toxic industrial materials, such as irritants, heavy metals, radioactive metals, acids and acid irritants, pesticides, and various agricultural chemicals, (collectively referred to as toxic chemical, materials, or simply toxins) as well as decontaminating surfaces that have come into contact with these agents. As a result, the formulation of the present invention can be used for neutralizing a broad spectrum of toxic chemicals and materials. In one embodiment, the active ingredient comprises 2, 3, butanedione monoxime (also known as diacetyl monoxime (DAM)), and alkali salts thereof such as potassium 2,3-butanedione monoxime (KBDO). The formulation also typically includes a carrier in which the active ingredient is dispersed. In one embodiment, the carrier comprises polyethylene glycol (PEG); monomethoxypolyethylene glycol (mPEG); and combinations and derivatives thereof.

The present invention is directed to a formulation and associated method for neutralizing one or more toxic chemical and/or materials including toxic industrial chemicals and toxic industrial materials, such as irritants, heavy metals, radioactive metals, acids and acid irritants, pesticides, and various agricultural chemicals, (collectively referred to as toxic chemical, materials, or simply toxins) as well as decontaminating surfaces that have come into contact with these agents. As a result, the formulation of the present invention can be used for neutralizing a broad spectrum of toxic chemicals and materials. In one embodiment, the active ingredient comprises 2,3, butanedione monoxime (also known as diacetyl monoxime (DAM)), and alkali salts thereof such as potassium 2,3-butanedione monoxime (KBDO). The formulation also typically includes a carrier in which the active ingredient is dispersed. In one embodiment, the carrier comprises polyethylene glycol (PEG); monomethoxypolyethylene glycol (mPEG); and combinations and derivatives thereof.

The present invention relates to isolated polypeptides having organophosphorous hydrolase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Disclosed herein are compositions, methods, and articles of personal protective equipment for use in detoxifying an organophosphate-based agent, wherein the compositions, methods, and articles comprise contacting the organophosphate-based agent with a cocaine esterase, wherein the contacting detoxifies the organophosphate-based agent.

The present invention is directed to methods of eluting protoporphyrinogen oxidase inhibitors from agricultural equipment comprising contacting the agricultural equipment with a composition comprising one or more amines. The present invention is further directed to compositions for eluting protoporphyrinogen oxidase inhibitors from agricultural equipment comprising one or more amines. The present invention is further directed to methods of degrading protoporphyrinogen oxidase (PPO) inhibitors comprising contacting the PPO inhibitors with an effective amount of a composition comprising one or more amines. The present invention is further directed to compositions for degrading protoporphyrinogen oxidase inhibitors comprising an effective amount of one or more amines.