A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimised depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimised depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

This invention relates to a process for producing a genetically modified seed. In particular, there is provided a process for producing a genetically modified Cannabis seed that germinates into a plant, the process comprising: (a) preparing a cell culture comprising genetically engineered Cannabis cells having at least one gene that expresses a psychoactive cannabinoid deleted; (b) establishing a callus culture for forming a somatic embryo; (c) forming a bio-ink comprising the somatic embryo that is encapsulated by a hydrogel and used as an artificial seed; and (d) three-dimensional (3D) printing the artificial seed comprised in the bio-ink. Also provided the 3D-printed artificial Cannabis seed having a shape other than that of a naturally occurring wild type Cannabis seed.

This invention relates to a process for producing a genetically modified seed. In particular, there is provided a process for producing a genetically modified Cannabis seed that germinates into a plant, the process comprising: (a) preparing a cell culture comprising genetically engineered Cannabis cells having at least one gene that expresses a psychoactive cannabinoid deleted; (b) establishing a callus culture for forming a somatic embryo; (c) forming a bio-ink comprising the somatic embryo that is encapsulated by a hydrogel and used as an artificial seed; and (d) three-dimensional (3D) printing the artificial seed comprised in the bio-ink. Also provided the 3D-printed artificial Cannabis seed having a shape other than that of a naturally occurring wild type Cannabis seed.

The unique annual herbaceous cannabis plant variety ‘DRG3’ is provided. The variety can be distinguished by its outstanding features of a distinct “oranges and spice” flavor profile and a distinct purple subset leaf and flower colors.

The unique annual herbaceous cannabis plant variety ‘DRG3’ is provided. The variety can be distinguished by its outstanding features of a distinct “oranges and spice” flavor profile and a distinct purple subset leaf and flower colors.

The inventive technology relates to systems and methods for enhanced in vivo production, accumulation and modification of cannabinoids. In one embodiment, the invention may include systems and methods for enhanced in vivo biosynthesis of chemically-modified water-soluble cannabinoids in a whole plant, or a cell suspension culture system.

According to the invention, there is provided novel hemp Cannabis cultivars with THC content of 0.2% by dry weight and a unique terpene profile. The cultivars exhibit increased CBD content of greater than about 1.07% by weight. The plants also have increased plant height and decreased branching that allows for increased yields of hemp and CBD content per acre. This invention thus relates to the seeds of hemp Cannabis cultivars of the invention, to the plants of hemp Cannabis cultivars of the invention, to plant parts of hemp Cannabis cultivars of the invention, to methods for producing a Cannabis cultivar by crossing the hemp Cannabis cultivars of the invention with another Cannabis cultivar, and to methods for producing a Cannabis cultivar containing in its genetic material one or more backcross conversion traits or transgenes and to the backcross conversion Cannabis plants and plant parts produced by those methods.

Provided is a Cannabis plant exhibiting altered tetrahydrocannabinolic acid (THCA) and/or cannabidiolic acid (CBDA) content. The plant includes a modified genomic locus involved in tetrahydrocannabinolic acid synthase (THCAS) and/or cannabidiolic acid synthase (CBDAS) gene expression, the genomic locus includes at least one targeted nucleotide modification within a regulatory region modulating the expression of at least one allele of the THCAS and/or CBDAS genes. Further provided are methods for producing the aforementioned Cannabis plant.

The unique annual herbaceous Cannabis plant variety ‘FS-tp2’ is provided. The variety is a selection resulting from cross of female parent Cannabis sativa. hybrid ‘Pineapple Express’ and male parent Cannabis sativa hybrid ‘Holy Banana’. Selective breeding was used to obtain the desired morphological features described. The morphological features that allow this variety ‘FS-tp2’ to be distinguished from other C. sativa varieties are its outstanding features such as high leaflet count, with narrow leaves.