This disclosure pertains to markers and methods useful in identifying Cannabis plants that have a day length neutral phenotype, and methods of breeding plants having a day length neutral phenotype.

An method of generating and selecting mutant Cannabis plants through mutagenesis of isolated Cannabis plant cells includes subjecting plant parts of one or more Cannabis plants to a pectinase treatment to obtain living cells of the one or more Cannabis plants, suspending the living cells in a mutagenic solution comprising methane sulfonate (EMS) and dimethyl sulfoxide (DMSO) to obtain mutated Cannabis cells, centrifuging the mutated Cannabis cells to obtain pelleted cells, and providing the pelleted cells on culture media.

An method of generating and selecting mutant Cannabis plants through mutagenesis of isolated Cannabis plant cells includes subjecting plant parts of one or more Cannabis plants to a pectinase treatment to obtain living cells of the one or more Cannabis plants, suspending the living cells in a mutagenic solution comprising methane sulfonate (EMS) and dimethyl sulfoxide (DMSO) to obtain mutated Cannabis cells, centrifuging the mutated Cannabis cells to obtain pelleted cells, and providing the pelleted cells on culture media.

A method for generating new varieties of Cannabis plants with modified growth and cannabinoid phytochemical profiles includes subjecting plant parts of one or more Cannabis plants to pectinase digestion to release plant cells, centrifuging the released plant cells to obtain pelleted cells, and providing the pelleted cells on culture media. The pelleted cells are plated on a first culture media, which is a Murashige and Skoog or Gamborg B5 callus culture media.

A method for generating new varieties of Cannabis plants with modified growth and cannabinoid phytochemical profiles includes subjecting plant parts of one or more Cannabis plants to pectinase digestion to release plant cells, centrifuging the released plant cells to obtain pelleted cells, and providing the pelleted cells on culture media. The pelleted cells are plated on a first culture media, which is a Murashige and Skoog or Gamborg B5 callus culture media.

The unique annual herbaceous Cannabis plant variety ‘SW0’ is provided. The variety can be distinguished by its outstanding features.

The present invention discloses a modified Cannabis plant exhibiting enhanced resistance to powdery mildew (PM). The aforementioned modified Cannabis plant comprises a mutated Cannabis mlo1 (Csmlo1) allele comprising a genomic modification selected from an indel of 14 bp at position corresponding to position 12 of SEQ ID NO: 882, or a fraction thereof, or a nucleic acid insertion at position corresponding to position 104-105 of SEQ ID NO: 882, or a combination thereof. The present invention further discloses methods for production of the modified Cannabis plant using genome modification.

‘DKJ127’ is a Cannabis sativa cultivar suitable for production of herbal medicinal products (standardized extract) having high to very high THC content, about 17-20% THC eq.

According to the invention, there is provided novel hemp Cannabis cultivars with THC content of 0.2% by dry weight and a unique terpene profile. This invention thus relates to the seeds of hemp Cannabis cultivars of the invention, to the plants of hemp Cannabis cultivars of the invention, to plant parts of hemp Cannabis cultivars of the invention, to methods for producing a Cannabis cultivar by crossing the hemp Cannabis cultivars of the invention with another Cannabis cultivar, and to methods for producing a Cannabis cultivar containing in its genetic material one or more backcross conversion traits or transgenes and to the backcross conversion Cannabis plants and plant parts produced by those methods.

The disclosure relates to a new and distinct Cannabis Sativa L. plant named ‘Midnight’, having an almost equal ratio of Cannabidiol (CBD) concentration to Tetrahydrocannabinol (THC) concentration, (e.g., substantially close to 1), as illustrated and described herein.