The invention provides a method for separating a mammal early follicle to obtain a single oocyte and a single granulocyte thereof. The method is capable of separating a mammal early follicle to obtain an active single oocyte and a corresponding granulocyte thereof. The invention further provides a kit for obtaining a single oocyte and a single granulocyte thereof from a mammal early follicle.

The present invention relates to a method of increasing fertility, or reducing rate of decline in fertility, or restoring fertility, of a female subject, comprising administering to the subject an effective amount of an agent which elevates SIRT2 activity or SIRT2 expression, and to compositions and kits for increasing fertility, or reducing rate of decline in fertility, or restoring fertility.

The present invention provides a method for expanding PGC/PGCLC, including culturing PGC/PGCLC in the presence of a phosphodiesterase 4 (PDE4) inhibitor and/or cyclosporine A, further in the presence of forskolin, and a method for inducing oocytes from PGC/PGCLC, including culturing PGC/PGCLC in the presence of bone forming protein (BMP) and retinoic acid (RA).

One measurement method of pig two cell embryo volume. Pig is an important economic animal, the regulation of reproduction can not only provide reference for human reproductive physiology and pathology process research, but also can provide a theoretical basis for improving the reproductive performance of pig. However, the low fertility is a problem plaguing the industry to raise pig. One measurement method of pig two cell embryo volume, based on the figure of pig two cell embryo that was obtained by the microscope, and then measurement, making the regression curve and regression equation. Thus, simple camera and can get more accurate measurement, embryo volume. that is conducive for further research to improve the developmental potential of pig embryo, enhance the production efficiency, is a reliable method to identificatory high quality embryo. The invention is applied to the field of embryo engineering technology.

The disclosure relates to Bovine germplasm of Bos taurus variety HO840M003150607238. Included in the present disclosure are cells comprising the genome of Bovine variety HO840M003150607238 characterized by the presence of homozygous loci and spermatozoa obtained from said cells. Also provided by the present disclosure are tissue cultures of cells, animals obtained from said cells, and parts thereof, including F1 spermatozoa. The disclosure further provides for methods of breeding, selecting, and using the germplasm to improve existing commercial cattle herds generated from in vitro fertilization methods and progeny cattle obtained from in vitro fertilization and implantation and artificial insemination methods.

The present disclosure relates to methods for increasing telomere length in one or more human adult cells and/or increasing genome stability of one or more human adult cells, for example by contacting one or more human adult cells with an agent that increases expression of Zscan4 in the one or more human adult cells. Methods of treating a subject in need of telomere lengthening, treating a disease or condition associated with a telomere abnormality, of rejuvenating one or more human adult cells, of rejuvenating tissues or organs, and of rejuvenating a subject in need thereof, for example by contacting one or more human adult cells in the subject with an agent that increases expression of Zscan4, or by administering to a subject in need thereof, an agent that increases expression of Zscan4 is also provided.

The present invention relates to a process for in vitro spermatogenesis from male germinal tissue comprising conducting maturation of testicular tissue comprising germ cells in a bioreactor which is made of a biomaterial and comprises at least one cavity wherein the germinal tissue is placed, and recovering elongated spermatids and/or spermatozoa.

Provided is a method for culturing immature oocytes. The method can promote in vitro maturation of the immature oocytes, and specifically comprises using follicular cells and a culture medium for culturing same. The culture medium for culturing the follicular cells contains CNP or variants thereof or analogues thereof and an HDAC (histone deacetylase) inhibitor. Also provided are the in vitro maturation culture medium containing CNP or variants thereof or analogues thereof and the HDAC inhibitor, and related compositions thereof, and the use of the above medium, culture medium and compositions in the promotion of in vitro maturation of the immature oocytes.

The present invention provides methods and compositions to improve the efficiency of somatic cell nuclear transfer (SCNT) of human cells and the consequent production of human nuclear transfer ESC (hNT-ESCs). More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of human donor somatic cells prevents efficient human somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of hSCNT by exogenous or overexpression of the demethylase KDM4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases SUV39h1 and/or SUV39h2.

An integrated chip is provided and comprises systems for sperm sorting, oocyte incubation and fertilization and liquid perfusion. The sperm sorting system comprises an inlet, an outlet, a main channel and a branch channel. The main channel has a width which increases along a direction from the inlet toward the outlet. The branch channel is connected to the main channel. The oocyte incubation and fertilization system is connected to the branch channel and comprises upper and lower holes. The upper hole is larger than the oocyte to hold the oocyte to be fertilized by a sperm. The lower hole is smaller than the oocyte. The sorted sperms are introduced into the oocyte incubation and fertilization system through the branch channel, to fertilize with the oocytes in the upper holes. The liquid perfusion system is located below the lower holes and a liquid exchange is performed through the second holes.