The present invention relates to site-selective modification of polysaccharides at their reducing end by conjugation with a single aminoxy-Regioselective Addressable Functionalized Template (RAFT) peptide.

Disclosed herein are detergent compositions that comprise at least one (i) glucan derivative and/or (ii) oxidized polysaccharide derivative. A glucan derivative of (i) can be substituted with at least one organic group that comprises a carboxylic acid group or a sulfonate group, and the degree of substitution (DoS) of the glucan derivative with the organic group can be about 0.1 to about 3.0. An oxidized polysaccharide derivative of (ii) can be produced by contacting a polysaccharide derivative under aqueous conditions with at least one agent that is capable of oxidizing the polysaccharide derivative. The polysaccharide derivative for oxidation has a degree of substitution (DoS) up to about 3.0 with at least one organic group. Further disclosed are various applications and products employing detergent compositions.

A composition includes a target pharmaceutical or biological agent, a solution containing the target pharmaceutical or biological agent, and substrate that is soluble in the solution. The substrate is capable of being solidified via a solidification process and the solidification process causes the substrate to become physically or chemically cross-linked, vitrified, or crystallized. As a result of the solidification process, particles are formed. The target pharmaceutical or biological agent within the solution retains proper conformation to ultimately produce a desired effect.

Polysaccharides may be partially oxidized by oxidative opening of a monosaccharide unit while still retaining glycosidic bonds. Such polysaccharides may be further functionalized with an amine moiety at a site of oxidative opening. Polysaccharides that are partially oxidized and amine-functionalized in this manner may be combined with an aqueous liquid to form compositions suitable for stabilizing clays in clay-containing formations. Clay stabilization may promote reduced swelling of the clays in the presence of water.

Disclosed herein are embodiments of compounds, conjugates, and devices, such as columns comprising such compounds and/or conjugates, that can be used to identify, separate, and quantify post-translationally modified analytes. The disclosed compounds and conjugates can be used to discriminate between analytes, such as peptides, having different post-translation modifications, such as methylations, phosphorylations, acetylations, citrullinations, hydroxylations, nitrosylations, ADP-ribosylations, glycosylations, propionylations, butyrylations, crotonylations, 2-hydroxyisobutyrylations, malonylations, succinylations, formylations, ubiquitinations, neddylations, proline cis-trans isomerizations. In particular disclosed embodiments, the compounds and conjugates can be used to separate peptides having different degrees of methylation.

Compositions are disclosed herein comprising a graft copolymer that comprises: (i) a backbone comprising dextran that has been modified with about 1%-25% alpha-1,2 branches, and (ii) one or more alpha-1,3-glucan side chains comprising at least about 50% alpha-1,3 glycosidic linkages. Further disclosed are reactions for producing such graft copolymers, as well as their use in derivatives, films and various other applications.

In one aspect, the disclosure provides methods of distinguishing a glycosaminoglycan from one or more other components in a sample by subjecting the sample to size-exclusion chromatography using a mobile phase having a pH of 6.8 or lower. A mobile phase having a pH of 6.8 or lower is found to improve the separation of glycosaminoglycans from proteins during size exclusion chromatography. In some embodiments, improved separation is due to the low pH of the mobile phase causing elution of less dispersed fractions of the protein and/or glycosaminoglycan. In some embodiments, the overlap between protein and/or glycosaminoglycan fractions is reduced.

The invention relates to: a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an amino acid sequence; a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; a complex containing a substrate and a protein with dextran-saccharase activity a method for synthesizing dextrans; and dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.

The subject matter of the invention is dextrans which have between 95% and 99% of -1,6 glucosidic bonds, a weight-average molar mass M.sub.w at least equal to 0.710.sup.9 g.mol.sup.1, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.

Disclosed herein are compositions that comprise at least one oxidized polysaccharide derivative. Such an oxidized polysaccharide derivative can be produced by contacting a polysaccharide derivative under aqueous conditions with at least one agent that is capable of oxidizing the polysaccharide derivative. The polysaccharide derivative for oxidation has a degree of substitution (DoS) up to about 3.0 with at least one organic group. Further disclosed are methods of producing oxidized polysaccharide derivatives, as well as their use in various applications and products.