Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

Disclosed is a method for preserving high molecular weight DNA in biological tissue, comprising contacting for a period of time at a temperature the biological tissue with an aqueous solution comprising a compound having the structure: ##STR00001##

Disclosed is a method for preserving high molecular weight DNA in biological tissue, comprising contacting for a period of time at a temperature the biological tissue with an aqueous solution comprising a compound having the structure: ##STR00001##

This disclosure relates to methods of super-cooling of aqueous samples with or without biological samples. The methods involve, e.g., providing a container comprising the aqueous sample; applying an immiscible liquid phase of sufficient thickness to separate the aqueous sample from air; and cooling the aqueous sample to a temperature that is below 0 C.

A system for the measurement of cell-free DNA that comes off of a kidney pump (perfusate) prior to transplantation to evaluate the viability of the organ (quantification of donor injury) and the risk for primary graft dysfunction after transplantation. This use of cfDNA does not require sequencing just quantification. A device that would connect to a kidney pump and directly measure the cfDNA and analyze it.

A system for the measurement of cell-free DNA that comes off of a kidney pump (perfusate) prior to transplantation to evaluate the viability of the organ (quantification of donor injury) and the risk for primary graft dysfunction after transplantation. This use of cfDNA does not require sequencing just quantification. A device that would connect to a kidney pump and directly measure the cfDNA and analyze it.

Provided herein are constructs of micro-aggregate multicellular, minimally polarized grafts containing Leucine-rich repeat-containing G-protein coupled Receptor (LGR) expressing cells for wound therapy applications, tissue engineering, cell therapy applications, regenerative medicine applications, medical/therapeutic applications, tissue healing applications, immune therapy applications, and tissue transplant therapy applications which preferably are associated with a delivery vector/substrate/support/scaffold for direct application.

Provided herein are constructs of micro-aggregate multicellular, minimally polarized grafts containing Leucine-rich repeat-containing G-protein coupled Receptor (LGR) expressing cells for wound therapy applications, tissue engineering, cell therapy applications, regenerative medicine applications, medical/therapeutic applications, tissue healing applications, immune therapy applications, and tissue transplant therapy applications which preferably are associated with a delivery vector/substrate/support/scaffold for direct application.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.