Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

An aspect of the invention relates to a method for detecting amyloid aggregates (40) in blood. The method comprises providing a blood sample (20) comprising one or more red blood cells and performing an analysis of a surface layer (22) of the blood sample (20) by an atomic force microscope (10) to detect amyloid aggregates on the surface of the red blood cells and to image detected amyloid aggregates. Further aspects relate to a corresponding atomic force microscope and a corresponding computer program product.

Devices for sorting components (e.g., cells) contained in a liquid sample are provided. In certain aspects, the devices include a magnetic separation device and an acoustic concentrator device fluidically coupled to magnetic separation device. Aspects of the invention further include methods for sorting cells in a liquid sample, and systems, and kits for practicing the subject methods.

A differential emissivity imaging device for measuring evaporable particle properties can include a heated plate, a thermal camera, a memory device, and an output interface. The heated plate can have an upper surface oriented to receive falling evaporable particles. The evaporable particles have a particle emissivity and the upper surface has a plate surface emissivity. The thermal camera can be oriented to produce a thermal image of the upper surface. A memory device can include instructions that cause the imaging device to calculate a mass of the individual evaporable particle via heat conduction using a calculated surface area and an evaporation time.

Described herein are cartridges and devices for operating said cartridges for analyzing a biological sample, such as a blood or saliva sample. Also described herein is an impedance sensor for analyzing a biological sample. Further described herein are methods of determining a cell count or detecting an analyte in a biological sample, which can include transporting the biological sample through a sensor comprising a channel or pore; applying an electrical current or voltage to the channel or pore; detecting an impedance within the channel or pore; and determining a cell count or detecting the analyte based on the detected impedance. Also described herein is an electrowetting electrode array that is configured to transport aqueous solutions using low voltage, such as about 50 volts or less. Further described herein are methods of transporting an aqueous liquid using electrowetting electrodes.

A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.

A chip includes a micro-channel unit for hydraulically classifying cells in a blood sample. In a micro-channel unit, liquid flowing from a sub channel into a main channel pushes cells flowing in the main channel toward a side thereof on which a removal channel and a collection channel are disposed. Fluid containing non-nucleated RBCs among the pushed cells enters the removal channel, so that the non-nucleated RBCs are removed from a blood sample. A plurality of micro-channel units having the same patterns as each other are repeatedly stacked in a height direction. Inlets of the main channels, inlets of the sub channels, outlets of the removal channels, outlets of the collection channels, and outlets of the main channels, which are provided in the micro-channel units, are connected to respective pillar channels penetrating each of layers in a traversing manner.

Provided is a label-free, single biological cell dielectric spectroscopy method, including the steps of: translocating a biological cell through a micropore or channel embedded in a substrate and interfaced with a coplanar waveguide while the biological cell experiences at least one RF field of at least 700 MHz provided via an RF input port to the coplanar waveguide; performing a time domain measurement of at least one RF signal reflected from or transmitted to a device under test (DUT); and determining an amplitude change and a phase change based on the reflected or transmitted at least one RF signal due to the translocating biological cell to determine an internal state or a morphological state of the biological cell. Also disclosed herein are devices for performing the dielectric spectroscopy method.

The invention provides methods for assessing immune system function and status based on cellular analysis. Methods of the present invention involve obtaining mass properties of immune cells collected in a tissue or body fluid sample from a subject. Such mass properties may include the mass of one or more immune cells and/or changes in mass of such immune cells over a period of time. Such data is then used for determining a status of the subject's immune response, and subsequent diagnosis and treatment of an infection or immunological disease or dysfunction.