The present disclosure pertains to sample preparation devices useful for affinity capture and purification that include one or more internal structures that comprise a reservoir, a well, a fluid passageway, sorbent particles, and a filter element that blocks passage of the affinity sorbent particles, which sample preparation devices combine the attributes of both dispersive and flow through designs into a single sample preparation device. The present disclosure also pertains to kits that contain and methods that use such sample preparation devices.

The present disclosure pertains to sample preparation devices useful for affinity capture and purification that include one or more internal structures that comprise a reservoir, a well, a fluid passageway, sorbent particles, and a filter element that blocks passage of the affinity sorbent particles, which sample preparation devices combine the attributes of both dispersive and flow through designs into a single sample preparation device. The present disclosure also pertains to kits that contain and methods that use such sample preparation devices.

The present invention relates to a flow-through device comprising at least one separation column wherein a first packing component, which comprises particles of alumina and/or silica, and a second packing component, which comprises a powder of one or more hygroscopic salts are provided. The two packing components may be blended or layered in the device, which may comprise a single tube or a plurality of tubes arranged in a plate format, such as the wells of a multiwall plate or tubes in a rack. In addition, the invention relates to a method for removing one or more matrix components, such as pigments, from a biological sample, by passing said sample across a first packing component, which comprises particles of alumina and/or silica, and a second packing component, which comprises a powder of one or more hygroscopic salts.

The present invention describes a simple purification process for recombinant human serum albumin. The process results in highly purified protein with limited number of purification steps. The broth containing human albumin is clarified by centrifugation and microfiltration, diafiltered and captured by cation exchange chromatography by a process that allows 140-230 mg of albumin to be captured per mi of resin. Product related impurities are removed by hydrophobic interaction chromatography, optimised to allow 87-97% recovery in flow through mode. The final series of processes are so combined that there is easy transition from one step to the next with minimal interventions and adjustments. The entire process of purification is completed within two days from harvest to final product. Thus a cost-effective process with improved recovery of protein at each step is developed. The purified human serum albumin is analyzed for purity and shows physicochemical characteristics that are similar to standard albumin.

A process for isolating astatine includes (a) contacting a composition comprising astatine and bismuth with nitric acid to form a first solution comprising astatine, bismuth, and nitric acid; (b) contacting a resin with the first solution so that astatine partitions out of the first solution and into the resin; and (c) eluting astatine from the resin. A composition comprising astatine may be of the formula AtO.sup.+X.sup.−, wherein X.sup.− is a counterion.

A process for isolating astatine includes (a) contacting a composition comprising astatine and bismuth with nitric acid to form a first solution comprising astatine, bismuth, and nitric acid; (b) contacting a resin with the first solution so that astatine partitions out of the first solution and into the resin; and (c) eluting astatine from the resin. A composition comprising astatine may be of the formula AtO.sup.+X.sup.−, wherein X.sup.− is a counterion.

The present invention relates to a preparative chromatography system (200, 500, 800) and a chromatography process (400, 700) adapted to repetitive cycling of chromatography volumes. The system (200, 500, 800) comprises at least two upstream pumps (203a, 803a, 203b, 803b) and separate flow paths (220) from process liquid sources to the chromatography device (200, 500, 800). The system (200, 500, 800) is arranged to prime one flow path (220) with one process liquid while providing another process liquid to the chromatography device and thereby minimizing the hold-up volume of the system (200, 500, 800).

In a method of preparing a liquid solution by mixing ingredients according to a predetermined recipe, wherein at least one pair of species of the liquid solution is derived from a weak electrolyte and corresponds to an acid-base pair, the conductivity of the liquid solution is predicted by: (i) for each pair of species derived from a weak electrolyte, solving a respective equilibrium equation to calculate the actual molar concentration of each such species at equilibrium in the liquid solution, (ii) calculating for each ionic species of said plurality of species the molar conductivity by the formula:
Λ=Λ.sub.0−K×Sqrt(c) wherein Λ is the molar conductivity, Λ.sub.0 is the molar conductivity at infinite dilution, c is the concentration, and K is the Kohlrausch coefficient, and wherein K and Λ.sub.0 are predetermined values for K and Λ.sub.0 for each ionic species, (iii) calculating the conductivity κ for each ionic species by the formula:
κ=c×Λ and (iv) adding up the conductivities determined in step (iii) for the different ionic species to obtain a predicted conductivity of the liquid solution. A computer program product comprises instructions for causing a computer to perform the method steps.

In a method of preparing a liquid solution by mixing ingredients according to a predetermined recipe, wherein at least one pair of species of the liquid solution is derived from a weak electrolyte and corresponds to an acid-base pair, the conductivity of the liquid solution is predicted by: (i) for each pair of species derived from a weak electrolyte, solving a respective equilibrium equation to calculate the actual molar concentration of each such species at equilibrium in the liquid solution, (ii) calculating for each ionic species of said plurality of species the molar conductivity by the formula:
Λ=Λ.sub.0−K×Sqrt(c) wherein Λ is the molar conductivity, Λ.sub.0 is the molar conductivity at infinite dilution, c is the concentration, and K is the Kohlrausch coefficient, and wherein K and Λ.sub.0 are predetermined values for K and Λ.sub.0 for each ionic species, (iii) calculating the conductivity κ for each ionic species by the formula:
κ=c×Λ and (iv) adding up the conductivities determined in step (iii) for the different ionic species to obtain a predicted conductivity of the liquid solution. A computer program product comprises instructions for causing a computer to perform the method steps.

A two-stage filtering system including a first and second filter container. The first filter container has a first filter assembly with a foam filter sleeve enveloping a fluid intake device, a connected valve, and transfer tubing. The second filter includes a pump connected to a spout, a second stage splashguard strainer, a second stage cup filter, and a second filter assembly. The second filter assembly includes at least one main filter comprising at least one carbon body filter enveloping a filter chamber, and exit tubing. The first filter container is structured to stack on top of the second filter container and the transfer tubing is structured to transfer first stage filtered fluid to the second filter container. The pump is structured to draw second stage filtered fluid from the second container through the exit tubing and expel the second stage filtered fluid out the spout to provide purified water.