The present disclosure relates to methods of modifying complex extracts such that components or mixtures of components are selectively removed or added, thus providing a complex mixture that does not naturally occur with a refined or a tuned therapeutic or nutraceutical effect. In various aspects, the complex extract can be an extract obtained from one or more plants, e.g., an extract obtained from green tea leaves. The present disclosure pertains to compositions obtained by the disclosed methods, nutraceutical compositions comprising same, pharmaceutical compositions comprising same, and methods of treating various conditions, including physiological dysfunctions associated with elevated reactive oxygen species and/or inflammatory molecule, e.g., TNFα, expression using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

A method for recycling polyester fabrics with use of an ionic liquid catalyst is provided, which includes: providing a recycled polyester fabric; and using a chemical de-polymerization liquid to chemically de-polymerize the recycled polyester fabric and form a de-polymerization product that includes bis-2-hydroxylethyl terephthalate (BHET). The chemical de-polymerization liquid is used to chemically de-polymerize the recycled polyester fabric in an environment where a de-polymerization catalyst exists, and the de-polymerization catalyst is the ionic liquid catalyst in a solid state.

A method for improving hue of recycled bis-2-hydroxylethyl terephthalate by using ionic liquids including providing a recycled polyester fabric; using a chemical de-polymerization liquid to chemically de-polymerize the recycled polyester fabric to form a de-polymerization product; mixing the de-polymerization product with water to form an aqueous phase liquid; dispersing an ionic liquid impurity adsorption material into the aqueous phase liquid to adsorb impurities originally present in the recycled polyester fabric.

A sample separation device for separating a fluidic sample includes a fluid drive for driving a mobile phase and the fluidic sample when injected in the mobile phase, a sample separation unit for separating the fluidic sample in the mobile phase, and a control unit configured for bracketing the fluidic sample between two mobile phase portions of the mobile phase. At least one of the mobile phase portions is arranged directly next to the fluidic sample and has a higher solvent strength compared to a solvent of the fluidic sample.

Buffer generators are described based on electrodialytic devices. The methods of using these devices can generate buffers for diverse applications, including separations, e.g., HPLC and ion chromatography. Also provided are chromatographic devices including the buffer generators, generally located upstream from a chromatography column, sample injector valve or both.

The degassing device includes a degassing flow path, a vacuum chamber, a vacuum pump, an inlet flow path, an outlet flow path, a drain flow path, a downstream side switching unit, and a controller. The degassing flow path is made of a gas-permeable, liquid-impermeable tube, and is accommodated in the vacuum chamber. The inlet flow path is for introducing a mobile phase to the degassing flow path, and the outlet flow path is for causing a mobile phase which has passed through the degassing flow path to flow out. The drain flow path is provided separately from the outlet flow path and is configured to drain the mobile phase in the degassing flow path from the degassing flow path. The downstream side switching unit is configured to switch the downstream end of the degassing flow path so as to be connected to either the outlet flow path or the drain flow path. The controller controls the operation of the downstream side switching unit, and is configured to connect the downstream end of the degassing flow path to the drain flow path at a predetermined timing when feeding of the mobile phase by the liquid feeding pump is stopped to drain the mobile phase in the degassing flow path through the drain flow path.

There is provided a method of operating a liquid chromatography arrangement, the liquid chromatography arrangement comprising: a solvent pump arranged to flow a liquid solvent over a liquid chromatography column; a restrictor arranged to restrict the liquid solvent from leaving the liquid chromatography column; and a liquid pump arranged to provide liquid flow between the liquid chromatography column and the restrictor, the method comprising: flowing the liquid solvent through the liquid chromatography column using the solvent pump; and controlling a liquid pressure within the liquid chromatography column by providing a liquid flow between the liquid chromatography column and the restrictor using the liquid pump.

A method for removing coloration from a drug substance solution of protein preparation, in particular, antibody preparation, a method for preparing drug substance solution of protein preparations including it, as a part thereof, and highly concentrated, colorless drug substance solutions thereof are disclosed. The method removes terminal glycation products causing the coloration in the drug substance solution of protein preparation, in particular, antibody preparation, by anion-exchange chromatography, making it possible to provide colorless drug substance solutions.

Buffer generators are described based on electrodialytic devices. The methods of using these devices can generate buffers for diverse applications, including separations, e.g., HPLC and ion chromatography. Also provided are chromatographic devices including the buffer generators, generally located upstream from a chromatography column, sample injector valve or both.