Certain embodiments of the invention provides a method for monitoring level of saturation of a chromatography media in a column, which method comprises measuring a first pressure at the inlet of an unloaded column; measuring a second pressure at the inlet from a loaded column; and comparing the first and second pressure measurement to determine the level of saturation of the chromatography media. Embodiments of the invention also provide related methods for controlling a chromatography system and methods for controlling a periodic counter current chromatography system, as well as a chromatography system suitable for use with the novel methods.

New metrics are disclosed for characterizing polyethylene copolymers which can be computed from the Cross-Fractionation Chromatography data of these polymers. These metrics are able to quantify the Broad Orthogonal Composition Distribution (BOCD) character of the polymers, and they can be used to discriminate polymers with an enhanced BOCD character from polymers that have the BOCD character to a lesser extent or from polymers that have the conventional molecular weight distribution and/or branching distribution.

Provided is a chiller and system that may be utilized in a supercritical fluid chromatography method, wherein a non-polar solvent may replace a portion or all of a polar solvent for the purpose of separating or extracting desired sample molecules from a combined sample/solvent stream. The system may reduce the amount of polar solvent necessary for chromatographic separation and/or extraction of desired samples. The system may incorporate a supercritical fluid chiller, a supercritical fluid pressure-equalizing vessel and a supercritical fluid cyclonic separator. The supercritical fluid chiller allows for efficient and consistent pumping of liquid-phase gases employing off-the-shelf HPLC pumps. The pressure equalizing vessel allows the use of off-the-shelf HPLC column cartridges. The system may further incorporate the use of one or more disposable cartridges containing silica gel or other suitable medium. The system may also utilize an open loop cooling circuit using fluids with a positive Joule-Thompson coefficient.

Exemplary embodiments are directed to devices, methods and systems capable of pressurization, generally involving a flow system that includes a pressurized reservoir, at least one pump including a pump control valve, an outlet port, a shut-off valve and a vent valve. The flow system is configured to be pressurized. The shut-off valve is disposed between the pressurized reservoir and the at least one pump. The vent valve is disposed between the at least one pump and the outlet port. The shut-off valve, the vent valve and the pump control valve of the at least one pump are configured to actuate in a coordinated manner to control a pressurization of the flow system. Exemplary embodiments are further directed to devices, methods and systems for column switching, generally including at least a first column, a second column and a column switching valve.

A method for separating at least two metals from each other in a metal refining process, the method comprising: injecting a feed solution comprising the metals into a column or flow pipe comprising a monolithic solid body having a plurality of channels; and flowing the feed solution through the plurality of channels in the monolithic solid body to separate the metals.

An object of the present invention is to suppress the variation of the elution position of a compound having a charged portion by a preservation liquid, in the purification of the compound, without carrying out the substitution step of the preservation liquid attached to the adsorbent used for the purification and the keeping step. A method for purifying a compound having a charged portion, the method comprising the steps of: preparing a composition containing a compound having a charged portion; preparing a buffer solution comprising a buffering agent and an alcohol, the buffer containing a calcium phosphate compound at least partially, having a buffer capacity in a range of pH 6.0 to pH 8.0, and being soluble in a polar solvent and insoluble in a non-polar solvent; preserving an adsorbent in the buffer solution; adsorbing the compound on the adsorbent by bringing the composition into contact with the adsorbent preserved in the buffer solution; and separating the compound from the adsorbent by gradient elution.

A liquid chromatography monitoring system comprises a computer or electronic controller comprising computer-readable instructions operable to: (a) draw a fluid into a syringe pump; (b) configure a valve so as to fluidically couple the pump to either a fluidic pathway through a fluidic system or to a plug that prevents fluid flow; (c) cause the syringe pump to progressively compress the fluid therein or expel the fluid to the fluidic pathway, while measuring a pressure of the fluid; (d) determine a profile of the variation of the measured pressure; (e) compare the determined profile to an expected profile that depends upon the fluid; and (f) provide a notification of a sub-optimal operating condition or malfunction if the determined profile varies from the expected profile by greater than a predetermined tolerance.

A liquid chromatography monitoring system comprises a computer or electronic controller comprising computer-readable instructions operable to: (a) draw a fluid into a syringe pump; (b) configure a valve so as to fluidically couple the pump to either a fluidic pathway through a fluidic system or to a plug that prevents fluid flow; (c) cause the syringe pump to progressively compress the fluid therein or expel the fluid to the fluidic pathway, while measuring a pressure of the fluid; (d) determine a profile of the variation of the measured pressure; (e) compare the determined profile to an expected profile that depends upon the fluid; and (f) provide a notification of a sub-optimal operating condition or malfunction if the determined profile varies from the expected profile by greater than a predetermined tolerance.

A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.