Aspects of the present disclosure relate to a method of regenerating a hydrophobic interaction chromatography column to which a load mass has been applied, the method comprising passing one or more column volumes of an alkaline solution through hydrophobic interaction media within the column, wherein the alkaline solution exhibits a pH of between about 10 and about 14, and a conductivity of between 0.5 mS/cm and about 10 mS/cm, wherein material bound to the hydrophobic interaction media is removed. In some cases, the alkaline solution may include sodium hydroxide at a concentration of between, e.g., about 0.1 mM and 10 mM.

The present disclosure relates to methods of re-oxidizing an antigen-binding protein.

A process increases the concentration of normal paraffins in a feed stream comprising separating a naphtha feed stream into a normal paraffin rich stream and a non-normal paraffin rich stream. A naphtha feed stream may be separated into a normal paraffin stream and a non-normal paraffin stream. An isomerization feed stream may be taken from the non-normal paraffin stream and isomerized over an isomerization catalyst to convert non-normal paraffins to normal paraffins and produce an isomerization effluent stream. The isomerization effluent stream may be separated into a propane stream and a C4+ hydrocarbon stream optionally in a single column. The C4+ hydrocarbon stream may be recycled to the step of separating a naphtha feed stream.

The present invention relates to a novel polypeptide affinity ligand coupled to solid supports and affinity purification of IgG antibodies. The invention is comprised of (1) the design, generation, and purification of polypeptide ligands, (2) coupling of a polypeptide affinity ligand to a solid support matrix, (3) purification of IgG (polyclonal and monoclonal antibodies), and (4) cleaning and reuse of polypeptide supported solid matrix.

The present invention relates to a novel polypeptide affinity ligand coupled to solid supports and affinity purification of IgG antibodies. The invention is comprised of (1) the design, generation, and purification of polypeptide ligands, (2) coupling of a polypeptide affinity ligand to a solid support matrix, (3) purification of IgG (polyclonal and monoclonal antibodies), and (4) cleaning and reuse of polypeptide supported solid matrix.

Methods, systems, and techniques for removal of PFAS contaminants from contaminated soil or sediment are provided. Example embodiments provide a water-based ex-situ method and system at a site that utilizes particle size and particle density segregation; deagglomeration, attrition, and retention time and sequential contacts with purified water; a recirculating water system with continual water treatment, and additional modules for destructive treatment of concentrated PFAS. In an example embodiment, the water treatment system of an example PFAS contaminant removal system and process includes ion exchange resin filtration component to remove PFAS effectively.

Method for lowering aldehyde content in a mixture comprising (i) diethylene glycol (DEG) and/or triethylene glycol (TEG) and (ii) aldehyde are disclosed. An ion exchange resin is soaked in monoethylene glycol. The mixture comprising 5 to 200 ppm aldehyde is then flowed to make contact with the soaked ion exchange resin to produce a product comprising DEG and/or TEG and less than 15 ppm aldehyde.

Method for lowering aldehyde content in a mixture comprising (i) diethylene glycol (DEG) and/or triethylene glycol (TEG) and (ii) aldehyde are disclosed. An ion exchange resin is soaked in monoethylene glycol. The mixture comprising 5 to 200 ppm aldehyde is then flowed to make contact with the soaked ion exchange resin to produce a product comprising DEG and/or TEG and less than 15 ppm aldehyde.

Packed bed gel material cleaning vessel, has an internal processing volume, to contain the gel, delimited by a circumferential, axially extending, upright vessel wall at both axial ends sealed by a top vessel wall and an opposite bottom vessel wall, the internal processing volume is above 10 litre; sensors of the vessel monitor the filling level of the vessel. A bottom filter completely covers the vessel bottom wall A circumferential, axially extending, cylindrical vertical filter is provided a short radial distance, e.g. between 1 and 20 millimetre internally from, parallel and concentrically with, the upright vessel wall, providing a torus like flow gap concentrical with the upright vessel wall.

The invention relates to a Protein A chromatography step of a purification process for a therapeutic protein, wherein a load solution including the therapeutic protein is applied onto a Protein A chromatography medium. According to the invention, a solution comprising CaCl.sub.2 is used as a wash solution for the Protein A chromatography medium for enhancing the removal of lipases, in particular phospholipase B-like 2 (PLBL2). The invention is of particular interest for the purification of CHO-expressed antibodies, such as tanezumab.