A large number of kinds of azo compounds which are representative hazardous substances in fiber products are divided into two groups. The compounds included in the first group are detected by an MRM measurement by a tandem mass spectrometer unit (12) in a measurement section (10) while a two-liquid gradient elution under an acidic condition is performed in a liquid chromatograph unit (11), using an aqueous ammonium acetate solution as mobile phase A, and a mixture of acetonitrile and an aqueous ammonium acetate solution as mobile phase B. On the other hand, the compounds included in the second group are detected by an MRM measurement while a two-liquid gradient elution under a neutral or weakly basic condition is performed using an aqueous ammonium bicarbonate solution as mobile phase A and acetonitrile as mobile phase B. An exhaustive quantitative analysis for major azo compounds can be achieved by performing the two analyses for the same sample. An efficient test with a shortened analysis period can thereby be performed.

A technique for comprehensively analyzing bile acids, sterols, and hormones is achieved. A method for analyzing bile acids, sterols, and hormones includes: a step of separating a plurality of molecules selected from among bile acids, sterols, and hormones in a sample by reversed-phase liquid chromatography; and a step of ionizing the molecules that have been separated; and a step of detecting, through MS analysis, the molecules that have been ionized.

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as Coh fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid absorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

A method is provided for removing THC from raw Cannabis oil. Additionally, new compositions of Cannabis oil are provided. Further, a new method of obtaining a substantially pure cannabinoid is provided

The patent discloses a method for efficiently collecting and purifying Orobanche cumana (O. cumana) germination stimulants using aeroponic system and solid-phase extraction (SPE), including the following steps: (1) sunflower seeds germination, then planting sunflower seedlings in aeroponic device, and cultivating the sunflower seedlings in the aeroponic system; at the aeroponic stage, phosphorus-containing aeroponic nutrient solution is first used to cultivate the sunflower seedlings for 20 to 25 days, and phosphorus-free aeroponic nutrient solution is then used instead to subject the sunflower seedlings to starvation cultivation for 5 to 7 days; and (2) passing all nutrient solutions in the aeroponic device through an SPE cartridge for SPE to extract O. cumana germination stimulants. The obtained O. cumana germination stimulants are diversified, and have high concentration and purity.

The present invention relates to the use of redox agents for purification of the CRM 197 variant of diphtheria toxin. The invention further relates to multi-step purification of CRM 197 from bacterial fermentates.

Methods for assaying function and/or activity and/or potency of isomerohydrolase proteins are provided.

The invention is directed to a precise, accurate and economical method for the simultaneous quantification of amounts of a dissolved sartan and dissolved statin in a mixture containing at least one sartan and at least one statin.

A chromatography device is provided comprising a filter housing having an inlet and an outlet and defining a fluid flow path between the inlet and the outlet; a porous filter arranged in the filter housing across the fluid flow path, the filter comprising first porous filter element; and a second porous filter element in contact with the first porous filter element, wherein the first porous filter element comprises at least one anionic exchange (AEX) layer, and the second porous filter element comprises at least one hydrophobic interaction (HIC) layer. A method of purifying nucleic acid using the device is also provided.

A fast and accurate reverse-phase high pressure liquid chromatography (“RP-HPLC”) method for detecting amino acids in small volumes (e.g. less than 50 .Math.L) of a biological sample, such as plasma. An assay for the simultaneous determination of ammonium and primary amino acids using RP-HPLC in samples such as plasma. A method for calculating intercellular volumes from a cell lysate to which a known volume and concentration of a non-naturally occurring amino acid is added.