A liquid chromatographic system includes a first cleaning pump that supplies a cleaning solution to a first valve, a selector valve connected to a first column and a second column, the selector valve switching an object to be connected to a detector that analyzes a separated sample between the first column and the second column, and a controller. The controller can have the first cleaning pump driven to clean the selector valve with the cleaning solution that flows through a first analysis flow path.

A liquid chromatographic system includes a column, a needle, a valve, a pump, and an analysis flow path. The valve sets the analysis flow path by switching between a first flow path through which an eluent flows from the valve to the column via the needle and a second flow path through which the eluent flows from the valve to the column not via the needle. The first flow path can be cleaned by drive of a first cleaning pump while the first flow path is set, and the second flow path can be cleaned by drive of the first cleaning pump while the second flow path is set.

The disclosure provides systems and methods useful for predicting or detecting a malfunction in a chromatography process in real-time. In some embodiments, the disclosure provides systems and methods for detecting an atypical profile in a process chromatogram in ion-exchange chromatography of a biologic product.

A simulated moving-bed type chromatographic separation method including separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component that has adsorptive property intermediate between the two components, with respect to an adsorbent, with two or more kinds of eluents by using a circulation system in which a plurality of unit packed columns each packed with an adsorbent are connected in series and in an endless form via pipes, the weakly adsorptive component, the strongly adsorptive component, and the intermediately adsorptive component being contained in a feed solution, wherein a feed solution supply port, eluent suply ports and a plurality of extraction ports are provided on the pipes of the circulation system, and positions of the feed solution supply port and the plurality of extraction ports are set to have a specified relationship; and a chromatographic separation system suitable for implementing the chromatographic separation method.

The invention provides processes of purifying a peptide including a GCC agonist sequence selected from the group consisting of SEQ ID NOs: 1-251 described herein. The processes include a solvent exchange step before a freeze-drying (lyophilization) step.

A chromatographic separation method for separating plural components contained in a liquid to be separated by a chromatography, with a separating device 1 including plural filling portions 10 filled with a separating agent for separating the plural components contained in the liquid to be separated, a supply portion 20 provided in each of the plural filling portions 10 to supply the liquid to be separated or an eluent for extracting any component contained in the liquid to be separated to the filling portion 10, and an extraction portion 30 to extract any component contained in the liquid to be separated from the filling portion 10, the method including: an upward supplying and extracting step of extracting any component contained in the liquid to be separated from an upward stream extraction portion while supplying the eluent to at least one filling portion 10 from an upward stream supply portion by an upward stream.

A method for producing 225.sup.A including: a method (X) for purifying a .sup.226Ra-containing solution, including an adsorption step of allowing a .sup.226Ra ion to adsorb onto a carrier having a function of selectively adsorbing a divalent cation by bringing a .sup.226Ra-containing solution into contact with the carrier under an alkaline condition, and an elution step of eluting the .sup.226Ra ion from the carrier under an acidic condition; a method for producing a .sup.226Ra target, including an electrodeposition liquid preparation step of preparing an electrodeposition liquid by using a purified .sup.226Ra-containing solution obtained by the method (X), and an electrodeposition step of electrodepositing a .sup.226Ra-containing substance on a substrate by using the electrodeposition liquid; and a step of irradiating a .sup.226Ra target produced by the method for producing a .sup.226Ra target with at least one selected from a charged particle, a photon, and a neutron by using an accelerator.

##STR00001##

The present invention relates to compounds of general formula (I), wherein —X is selected from a group consisting of H; C.sub.1 to C.sub.6, alkyl; halogen (F, Cl, Br or I); Y is selected from a group consisting of nitrogen; N-oxide; Z.sup.1, Z.sup.2, Z.sup.m, wherein m is 1 or 2, are independently selected from the group consisting of —CH.sub.2—CH.sub.2— and —CH.sub.2—CH.sub.2—CH.sub.24 A, A.sup.m, wherein m is 1 or 2, are independently selected from H; —CH.sub.2COOH; —CH.sub.2C(O)NH.sub.2; —CH.sub.2P(O)(OH).sub.2, and n is 1 or 2; R.sup.1, R.sup.2, R.sup.3 are independently H; C.sub.1 to C.sub.6, alkyl; C.sub.1 to C.sub.6 alkyloxy; C.sub.6 to C.sub.10 aryloxy; benzyloxy; C.sub.1 to C.sub.6 alkylthio; C.sub.6, to C.sub.10 arylthio; F; Cl; Br; I; OH; SH; NH.sub.2; C.sub.1 to C.sub.6, alkylamino; di(C.sub.1 to C.sub.6, alkyl)amino; C.sub.1 to C.sub.6 acylamino; di(C.sub.1 to C.sub.6 acyl) amino; C.sub.6 to C.sub.10 arylamino; di(C.sub.6 to C.sub.10 aryl)amino; CN; OH; nitro; COOR.sub.n, C(O)NHR.sub.n, C(O)N(R.sub.n).sub.2, wherein R.sub.n is independently H or C.sub.1 to C.sub.10 alkyl or C.sub.6, to C.sub.10 aryl; and/or neighboring two of R.sup.1, R.sup.2, R.sup.3 together with neighboring two carbon atoms of the aromatic cycle form a six-membered ring, optionally substituted with one or more substituents independently selected from the group consisting of OH, SH, CF.sub.3, F, Cl, Br, I, C.sub.1 to C.sub.6, alkyl, C.sub.1to C.sub.6, alkyloxy, C.sub.1 to C.sub.6 alkylthio, NH.sub.2, C.sub.1 to C.sub.6 alkylamino, di(C.sub.1 to C.sub.6 alkyl)amino, NO.sub.2, COOH, COOR.sub.n, C(O)NHR.sub.n, C(O)N(R.sub.n).sub.2, wherein R.sub.n is WO 2020/244686 A1 independently H or C.sub.1 to C.sub.10 alkyl or C.sub.6 to C.sub.10 aryl; with the proviso that when n is 2 and all of Z.sup.1, Z.sup.2, Z.sup.m are —CH.sub.2—CH.sub.2—, then A is not —CH.sub.2COOH; for chromatographic separation of rare earth elements and/or s-, p- and d-block metals, as well as to the method of the separation of rare earth elements.

The present disclosure is directed to method (100) for separating a biomolecule from a fluid (2), wherein the biomolecule is produced by biological cells in a perfusion bioreactor (3), the method comprising: forwarding (110) a product fluid comprising the biomolecule and biological cells from the perfusion bioreactor (3) during a bioreactor product output time period; loading (210) a first separation device (4) with the product fluid obtained in step (110) during a first separation loading time period, which constitutes a first part of a first separation cycle time period; pausing (130) the forwarding of the product fluid from the perfusion bioreactor during a bioreactor non-product output time period; and pausing (220) the loading of the first separation device (4) during a second part of the first separation cycle time period. Also disclosed are a computer-implemented method performed by a controller configured to control a separation of a biomolecule from a fluid (2), and further a controller, a computer program, and a computer program product.

This application relates to methods for the production of polysaccharide antigen-carrier protein conjugates, in particular conjugates of Group B Streptococcus (GBS) capsular polysaccharide and a carrier protein, in particular CRM197. The methods comprise a hydroxyapatite chromatography step in order to separate the conjugate from free polysaccharide and free carrier protein.