The present invention provides for simple and rapid filtering of biological samples, whereby a sample can be analyzed in the same device or a different device. In one preferred example, a membrane filter 12 that is particularly useful for the filtration of samples comprising viruses along with other biological materials that need be separated from the viruses is used. A lateral flow device 10 incorporating a filter membrane/membrane filter 12 is also disclosed.

There are provided a porous composite membrane formed by blending perfluoroalkoxy alkane (PFA) with an organic substance, and a manufacturing method thereof. The porous composite membrane is able to have pores easily formed simply by blending a fluorine-based polymer with an organic substance without additional pore-forming processes such as stretching and heating, and exhibit excellent properties in terms of resistance to high temperatures and strong acids due to the use of the fluorine-based polymer as a base material, so it is available for use in semiconductor wastewater treatment that uses strong acids like HF.

A hydrosol preparation system includes a conveyance unit, a distillation unit, a sterilization unit, and a filling unit. The conveyance unit is for conveying a container. The distillation unit performs distillation on a raw material to produce a crude hydrosol. The sterilization unit performs sterilization on the crude hydrosol to produce a final hydrosol. The filling unit fills the final hydrosol into the container. The preparation system integrates all procedures required by manufacturing of the hydrosol product so as to eliminate the need of transferring the semifinished hydrosol product among different production sites, thereby minimizing the risk of inclusion of impurities and maximizing safety and storability of the finished hydrosol product.

A method for exosome separation and extraction by stacked centrifugal filtration. It is used in molecular biology and clinical examination and comprises an exosome separation and extraction kit consisting of the stacked centrifugal filtration device, incubation buffer and protease K. The sample to be tested is incubated at room temperature using the incubation buffer and an appropriate amount of protease K, followed by centrifugation with the stacked centrifugal filtration device. After mixing thoroughly, the retentate in the ultrafiltration tube is collected to obtain the exosomes. The method needs no ultracentrifugation equipment and allows quick acquisition of purified exosomes within 30 min. This method is suitable for clinical purification for exosome tests, featuring by its short operating time and the ability for parallel treatment of multiple samples, with reasonable cost.

[Object]

To provide an isolation and purification method for isolating and purifying extracellular vesicles.

[Solution]

An isolation and purification method of extracellular vesicles is disclosed. A hollow fiber membrane has an inner diameter of 0.2 mm to 1.4 mm and a molecular weight cut-off of 100000 to 1000000. Filtering includes a first filtration process of press-fitting the culture supernatant of the mesenchymal stem cells from a first opening on one end side of the hollow fiber membrane and filtering the culture supernatant to separate the culture supernatant into a permeate and a first concentrate, and a second filtration process of press-fitting the first concentrate from a second opening on the other end side of the hollow fiber membrane and filtering the first concentrate to separate the first concentrate into a permeate and a second concentrate. A concentrate is produced in which a concentration of the extracellular vesicles is increased by alternating tangential flow filtration in which the first filtration process and the second filtration process are alternately performed a plurality of times. A membrane surface velocity in the first filtration process and the second filtration process is 0.3 m/sec to 2 m/sec.

A method is provided for using hollow fibers having a porosity above 20 nm, in particular polyethersulfone hollow fibers, to impoverish blood and blood-derivatives from blood-derived extracellular vesicles, in particular exosomes and exomers. Methods for obtaining and analyzing the impoverished samples are also provided.

A ceramic membrane filter includes a porous substrate including cells through which a fluid flows, an intermediate membrane formed on the porous substrate, and a separation membrane formed on the intermediate membrane. In this ceramic membrane filter, the percentage of the number of cells having cracks with a size of 4 ?m or less relative to the total number of cells is 9% or less.

Embodiments of the present disclosure provide a method for preparing a high-purity arabinose crystal, comprising operations of dissolving, blending, ion exchange, decolorization and filtration, fine filtration, evaporation and concentration, crystallization, centrifugation, and drying performed in sequence on a low-purity arabinose crystal. Throughout the operations of preparing the arabinose crystal, a pH value of a material may be controlled between 4.3 and 7.5, and a temperature may not exceed 70? C. This prevents arabinose from transforming into impurities under a high temperature, thereby maintaining and improving a purity of the prepared arabinose crystal.

Disclosed are a preparation method, a product and an application of a hydrophobically modified membrane based on multi-effect thermal energy conversion, the preparation method includes the steps: S1. dispersing carbon nanotubes with surfaces carboxylated in a solvent to form a dispersion; S2. applying the dispersion evenly on a PVDF membrane, and drying to form a ready-to-use membrane; S3. performing thermo-mechanical pressure treatment of the ready-to-use membrane to form a functional membrane with strong robustness; and S4. placing the functional membrane with strong robustness in an alkane solution of PDMS containing a silane coupling agent, and then taking it out for drying.

In a microporous layer of the present embodiment, a falling angle of an aqueous solution containing 25 wt % of ethanol is less than 30? and a strength determined by SAICAS evaluation is more than 0.068 N.