A method for encapsulating active ingredients in liposomes having an active ingredient solution encapsulated with a bilayer composed of two monomolecular layers of amphiphilic compounds comprises: (a) providing the active ingredient solution; (b) providing an emulsion by emulsifying the active ingredient solution in a first liquid in the presence of the amphiphilic compound; (c) providing a liquid phase; (d) contacting the emulsion with the liquid phase to form a phase boundary; and (e) centrifuging the emulsion and the liquid phase that are in contact with one another via the phase boundary, wherein, on passage of the phase boundary, the amphiphilic compound enriched there is added onto the monomolecular inner layer to form a monomolecular outer layer, in order to create the bilayer.
The first liquid of the emulsion is chosen such that the solubility of the amphiphilic compound in the first liquid is not more than 1×10.sup.−4 mol/l.

Producing a resin particle dispersion using an apparatus including: two or more resin particle dispersion production lines each including an emulsification tank in which a resin is subjected to phase inversion emulsification using two or more organic solvents and an aqueous medium to obtain a phase-inverted emulsion, a distillation tank in which the organic solvents are removed from the phase-inverted emulsion by reduced pressure distillation to obtain a resin particle dispersion, and plural distillate collection tanks that collect distillates formed during the reduced pressure distillation according to respective target distillate compositions; and a reusable storage tank that collects and stores a distillate collected in at least one collection tank among the distillates collected in the plural collection tanks in each of the two or more production lines, and delivering the distillate to the emulsification tank in at least one production line to reuse the distillate for producing a phase-inverted emulsion.

Various examples of systems and methods for making microspheres, microparticles, and emulsions are provided. In one example, a system and method for forming microspheres comprises: pumping a dispersed phase liquid and a continuous phase liquid into a levitating magnetic impeller pump to subject the dispersed phase liquid and continuous phase liquid to a high shear environment within the impeller pump's pump chamber. In another example, a system and method for forming an emulsion comprises: pumping a dispersed phase liquid and an inner aqueous phase liquid into a levitating magnetic impeller pump to subject the dispersed phase and the inner aqueous phase to a high shear environment within the impeller pump's pump chamber.

The present invention relates to a microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, the present invention relates to an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets. The microfluidic device comprises a transfer conduit comprising a first transfer conduit part having a first affinity for water; and a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water. A well section and a microfluidic section of the microfluidic device are fixedly connected to each other.

Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

Provided herein is a first fluid dispersed in a second fluid to form an emulsion of micro-droplets having an average droplet size and having a droplet size distribution around the average droplet size and below a maximum droplet size. The micro-droplets will lose their solvent to transform to micro-spheres exhibiting a particle size distribution around an average particle size and substantially below a maximum allowable particle size. The micro-spheres are subjected to a micro-filter having a relatively narrow pore size distribution around an average pore size, which average pore size is between the average particle size and the maximum particle size. A filtrate of the micro-filter comprises a majority of the micro-spheres that is substantially void of micro-spheres having a particle size exceeding the maximum allowable particle size.

Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

A flow focusing type one-step double emulsion droplet parallel generation device and method. The device comprises a fluid injection module, a liquid droplet generation module, a liquid droplet surface solidification module and a liquid droplet collection module, wherein the fluid injection module is used for conveying fluid of each phase to the liquid droplet generation module; and the liquid droplet generation module comprises a fluid distribution functional area, a liquid droplet preparation functional area and an auxiliary functional arca, wherein the liquid droplet distribution functional area is used for conveying the fluid of each phase into a channel of the fluid of each phase corresponding to the liquid droplet preparation functional area, and the fluid of each phase is gathered at the same point in a flow focusing structure and then is broken, and a fluid of an outer phase covers the fluid of the middle phase.

Embodiments of the present application relate to batches of bupivacaine multivesicular liposomes (MVLs) prepared by a commercial manufacturing process using independently operating dual tangential flow filtration modules.

Membrane Emulsification Apparatus with Refiner There is described a membrane emulsification apparatus for dispersing a first phase in a second phase, comprising: •a membrane defining a plurality of apertures connecting a first volume on a first side of the membrane to a second volume on a second, different, side of the membrane, the apparatus being arranged to receive a first phase containing a liquid in the first volume and to receive a second phase in the second volume the apparatus being adapted to generate an emulsion through egression of the first phase into the second phase via the plurality of apertures; •the apparatus also comprising a refiner (1) arranged to receive the emulsion from the membrane; and wherein said refiner comprises an inlet (4/5) and an outlet (5/4) wherein an opening (8) adapted to converge flow of the emulsion and to break up droplets of the emulsion into a refined emulsion is located between the inlet and the outlet.