The present invention provides porous materials for use in solid phase extractions and chromatography. In particular, the materials exhibit superior properties in the SPE analysis of biological materials. In certain aspects, the porous materials comprise a copolymer of a least one hydrophobic monomer and at least one hydrophilic monomer, wherein more than 10% of the BJH surface area of the porous material is contributed by pores that have a diameter greater than or equal to 200 Å, wherein said material has a median pore diameter of about 100 Å to about 1000 Å, or both. In some embodiments, the at least one hydrophilic monomer has a log P value of less than 0.5. In some embodiments, the at least one hydrophilic monomer is selected from 4-acryloymorphine, N-(3-methoxypropyl)acrylamide, N,N′-methylenebis(acrylamide), acrylonitrile, ethylene glycol dimethacrylate, methyl acrylate, 4-acetoxystyrene, 4-vinyl pyridine, or a boronic-acid-containing monomer, among others.

The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

The present disclosure provides devices, systems, kits and methods useful for quantitation of biomolecules such as intact proteins and nucleic acids.

An adsorbent composition comprises a bismuth material, a promoter and optionally a support. The adsorbent composition is suitable for adsorbing an arsenic material, such as arsine, from a process stream.

The invention concerns methods of removing undesirable molecules from the blood or physiologic fluid; said method comprising contacting said blood or physiologic fluid with a sorbent, said sorbent comprising a plurality of solid forms and comprising a cross-linked polymeric material having a plurality of ligands attached to the surface of said cross-linked polymeric material, comprising (i) zwitterionic moieties, (ii) oligo(ethylene glycol) moieties or (iii) mixtures thereof; said contacting comprising said sorbent sorbing a plurality of said undesirable molecules when said sorbent is administered within a patient's body.

A method for producing porous particles of a cross-linked polymer, and porous particles that can be produced according to the method are disclosed. The porous particles of a crosslinked hydroxy- or amino-group-containing polymer have a relatively low swelling factor. A composite material contains the porous particles dispersed in a continuous aqueous phase. The porous particles, or the composite material, are used for purifying organic molecules and for bonding metals from solutions. A filter cartridge contains the porous particles of a cross-linked polymer or the composite material.

A method of adsorbing a highly reactive gas onto an adsorbent material comprising adsorbing the highly reactive gas to the adsorbent material. The absorbent material comprises at least one Lewis basic functional group, or pores of a size to hold a single molecule of the highly reactive gas, or inert moieties which are provided to the adsorbent material at the same time at the same time as the highly reactive gas, prior to adsorbing the highly reactive gas or after adsorbing the highly reactive gas, or the highly reactive gas reacts with moieties of the adsorbent material resulting in passivation of the adsorbent material. A rate of decomposition of the adsorbed highly reactive gas is lower than a rate of decomposition for the neat gas at equal volumetric loadings and equal temperatures for both the adsorbed highly reactive gas and the neat gas.

A composite sorbent composition comprising a polymeric adsorbent; and an extractant having the formula, or a hydrate thereof, wherein Z is —C(O)— or —C(R′)(R″)— wherein R′ and R″ are each hydroxyl; and R.sup.1, R.sup.2, R.sup.3, and R.sup.4 are each independently hydrogen, halogen, hydroxyl, cyano, nitro, amino, —C(O)H, —C(O)OH, —C(O)NH.sub.2, alkyl, alkenyl, alkynyl, alkoxy, alkanoyl, alkylamino, alkyl carboxamide, alkyl ester, heteroalkyl, haloalkyl, haloalkoxy, cycloalkyl, aryl, arylalkyl, aryloxy, heterocycloalkyl, heteroaryl, heteroaryloxy, or heteroarylalkyl, each of which R.sup.1, R.sup.2, R.sup.3, and R.sup.4 is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, WO 2019/023355 A1 cyano, nitro, sulphonato, amino, —C(O)H, —C(O)OH, —C(O)NH.sub.2, alkyl, alkoxy, alkylamino, heteroalkyl, haloalkyl, haloalkoxy, cycloalkyl, aryl, arylalkyl, aryloxy, heterocycloalkyl, heteroaryl, heteroaryloxy, or heteroarylalkyl; or R.sup.1 and R.sup.2, R.sup.2 and R.sup.3, or R.sup.3 and R.sup.4 are together a group —O—CH.sub.2—O—, a group —CH.sub.2—O—CH.sub.2—, or a group —CH.sub.2—CH.sub.2—CH.sub.2—CH.sub.2—.

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Disclosed herein are extraction chromatographic supports comprising a porous support, an inert filler, and metal ion binding extractant that may be used for chromatographic separation of metal ions. Also disclosed herein are methods for preparing and using the extraction chromatographic supports.

Provided are a toxin separator and the like which are capable of selectively separating toxin present in a biological fluid by binding to protein, from the toxin and the protein. The toxin separator of the present invention also includes activated carbon of which a pore volume of pores having a pore diameter from 1.4 to 35 nm as measured by a nitrogen adsorption method is 0.06 cm.sup.3/g or greater.