The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

The invention concerns biocompatible polymer systems comprising at least one polymer with a plurality of pores, said polymer comprising either polyol or zwitterionic groups designed to adsorb endotoxins and other inflammatory mediator molecules. The inventions are in the field of porous polymeric sorbents, also in the field of broadly reducing endotoxins in blood and blood products that can cause endotoxemia, additionally, in the field of broadly removing endotoxins by perfusion or hemoperfusion.

There is provided a composite material comprising a porous silica particle, a plurality of metal particles disposed within the pores of said silica particle and a polymeric coating that at least partially encapsulates said silica particle. There is also provided a method of preparing a composite material, comprising the step of mixing a solution containing a plurality of activated metal and silica particles with a polymer solution to thereby form said composite material, wherein said composite material comprises a porous silica particle, a plurality of metal particles disposed within the pores of said silica particle and a polymeric coating that at least partially encapsulates said silica particle.

The present invention provides activated carbon with which hydrophilicity is excellent and the amount of steam adsorbed is increased, and provides a method for producing this activated carbon. This activated carbon is characterized in that the amount of basic functional groups in the activated carbon is 0.470 meq/m.sup.2 or greater. Preferably the amount of basic groups per specific surface area of activated carbon is 0.200 μeq/m.sup.2 or greater and the ratio of the amount of basic functional groups and the amount of acidic functional groups (basic functional groups/acidic functional groups) is 1.00 or greater. This method for producing activated carbon is characterized in comprising a step for imparting basic functional groups by bringing the activated carbon into contact with a basic substance. According to a preferred embodiment, the method comprises a step for heating the resulting activated carbon in an insert atmosphere.

The subject technology is directed to a CO.sub.2-based chromatography system and method for rapid determination of the levels and/or the presence or absence of steroids or steroid derivatives in a sample.

The rate of recovery of a purification target gas from a gas purification apparatus that uses a PSA device is improved, and both a high purity and a high recovery rate are achieved with good power efficiency. The present invention is directed to a gas purification method using the PSA method, in which a carbon molecular sieve having a pore volume, at a pore diameter of 0.38 nm or more, of not exceeding 0.05 cm.sup.3/g and a pore volume, at a pore diameter of 0.34 nm, of 0.15 cm.sup.3/g or more, in a pore diameter distribution measured by the MP method is used as an adsorbent, and, in an adsorption step, a miscellaneous gas is adsorbed from a source gas by bringing the source gas into contact with the adsorbent for 10 seconds or more and 6000 seconds or less so as to obtain a concentrated methane.

The invention concerns polymeric media based on modified natural polysaccharides for removing one or both of Anti-A Antibodies and Anti-B Antibodies from human blood or plasma, the media comprising one or both of (i) a polymeric solid support with a blood group A Antigen ligand attached to the solid support at a ligand loading between 1-5 mg/mL of solid support, and wherein the media is stable under physiological pH conditions, and (ii) a polymeric solid support with a blood group B Antigen ligand attached to the solid support at a ligand loading between 1-5 mg/mL of solid support, and wherein the media is stable under physiological pH conditions.

Provided herein are materials and methods of reducing contamination in a biological substance or treating contamination in a subject by one or more toxins comprising contacting the biological substance with an effective amount of a sorbent capable of sorbing the toxin, wherein the sorbent comprises a plurality of pores ranging from 50 Å to 40,000 Å with a pore volume of 0.5 cc/g to 5.0 cc/g and a size of 0.05 mm to 2 cm and sorbing the toxin. Also provided are kits to reduce contamination by one or more toxins in a biological substance comprising a sorbent capable of sorbing a toxin, wherein the sorbent comprises a plurality of pores ranging from 50 Å to 40,000 Å with a pore volume of 0.5 cc/g to 5.0 cc/g and a size of 0.05 mm to 2 cm and a vessel to store said sorbent when not in use together with packaging for same.

Compositions containing a porous organic polymer and a heavy metal chelating moiety are provided for binding heavy metals, for example in remediation and purification. The compositions can be stable and recyclable. The compositions can contain heavy metal chelating moieties such as a thiol, a sulfide, an amine, a pyridine, or a combination thereof. The compositions can bind heavy metals such as lead, cadmium, and mercury. The compositions can have a large surface area greater than about 20 m.sup.2/g. The compositions can be used for remediation and purification to remove heavy metals from a solution. The compositions can have a maximum metal uptake capacity of more than 500 mg g.sup.−1 and/or a metal distribution coefficient of at least 1×10.sup.7 mL g.sup.−1 at 1 atm and 296 K. Methods of making the compositions are provided. Methods of binding heavy metals in remediation and purification are also provided.

The present invention provides a column filler for liquid chromatography that has a great adsorption capacity, adjustable adsorption selectivity, and high shape retainability and therefore is usable for measurement of various substances and capable of achieving excellent separation performance and a high filling rate in a column when used as a column filler for liquid chromatography. Provided is a column filler for liquid chromatography including carbon-coated porous particles, the carbon-coated porous particles including porous particles each having a coating layer containing an amorphous carbon on a surface.