The invention relates to a method of chromatography wherein a gaseous, liquid or supercritical fluid mobile phase, which contains substances to be separated, flows through a porous packing which comprises a plurality of capillary channels which extend in the direction of flow of said mobile phase, said packing being manufactured by a method wherein: a bundle of elementary fibres is assembled, said fibres comprising a core made of a solid, liquid or gaseous material, and a shell made of a drawable material, said bundle is drawn in order to reduce the diameter of said fibres, a porous matrix is formed around the core of the drawn fibres, the formation of said porous matrix comprising a transformation of the shell material, where said porous matrix comprises at least one population of connected pores interconnecting the channels, where the thickness of the porous matrix between two adjacent channels is less than the diameter of the channels, preferably less than half the diameter of the channels, where necessary the core material is removed so as to leave free channels in the porous matrix.

The invention relates to a method of chromatography wherein a gaseous, liquid or supercritical fluid mobile phase, which contains substances to be separated, flows through a porous packing which comprises a plurality of capillary channels which extend in the direction of flow of said mobile phase, said packing being manufactured by a method wherein: a bundle of elementary fibres is assembled, said fibres comprising a core made of a solid, liquid or gaseous material, and a shell made of a drawable material, said bundle is drawn in order to reduce the diameter of said fibres, a porous matrix is formed around the core of the drawn fibres, the formation of said porous matrix comprising a transformation of the shell material, where said porous matrix comprises at least one population of connected pores interconnecting the channels, where the thickness of the porous matrix between two adjacent channels is less than the diameter of the channels, preferably less than half the diameter of the channels, where necessary the core material is removed so as to leave free channels in the porous matrix.

Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Provided is a novel method for producing cellulose beads, the method being capable of suitably producing spherical cellulose beads. The method for producing cellulose beads includes: (Step 1) preparing a coagulating bath having a first liquid medium phase and a second liquid medium phase; (Step 2) forming a particulate cellulose solution in the first liquid medium phase by supplying a cellulose solution to the first liquid medium phase of the coagulating bath; and (Step 3) coagulating the particulate cellulose solution by supplying the particulate cellulose solution in the first liquid medium phase to the second liquid medium phase.

Provided is a carrier which has excellent pressure resistance, and even when a protein ligand is not immobilized thereon, has a high dynamic binding capacity to a target substance, and has a high performance of separating a target substance from a biological sample.

The carrier includes a polymer having a crosslinked structure containing a divalent group represented by the following Formula (1):

##STR00001## wherein R.sup.1 to R.sup.4 independently represent a single bond or a divalent hydrocarbon group, R.sup.5 and R.sup.6 independently represent a hydrogen atom or a hydrocarbon group, X represents a thio group, a sulfinyl group, a sulfonyl group, an oxy group, >N(—R.sup.31), >Si(—R.sup.32).sub.2, >P(—R.sup.33), >P(═O)(—R.sup.34), >B(—R.sup.35), or >C(—R.sup.36).sub.2 (R.sup.31 to R.sup.36 independently represent a hydrogen atom or hydrocarbon group), and * represents a bond, with a proviso that when both R.sup.1 and R.sup.3 are a divalent hydrocarbon group, R.sup.1 and R.sup.3 may form a ring together with an adjacent carbon atom, and when both R.sup.2 and R.sup.4 are a divalent hydrocarbon group, R.sup.2 and R.sup.4 may form a ring together with an adjacent carbon atom.

Provided is a packing material for liquid chromatography, including a gel obtained by polymerizing monomers including 40% by mass or more of a crosslinkable monomer having a (meth)acryloyloxy group.

Provided is a packing material for liquid chromatography, including a gel obtained by polymerizing monomers including 40% by mass or more of a crosslinkable monomer having a (meth)acryloyloxy group.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution.