The present invention provides: a porous fiber that exhibits both improved adsorption capacity, and suppressed exposure and detachment of particulates; an adsorption column filled with said porous fiber; and a blood purification system in which an adsorption column is connected to a water removal column. The porous fiber according to the present invention has a three-dimensional pore structure formed by a solid fiber, and satisfies all of the following conditions. (1) The porous fiber has particulates having a diameter of not more than 200 μm, and the percentage of area occupied by said particulates having a diameter of not more than 200 μm in a horizontal cross section of the three-dimensional pore structure is at least 3.0%. (2) The porous fiber does not contain said particulates having a diameter of not more than 200 μm in the region within 1.0 μm in the depth direction from the outermost surface.

The present invention provides: a porous fiber that exhibits both improved adsorption capacity, and suppressed exposure and detachment of particulates; an adsorption column filled with said porous fiber; and a blood purification system in which an adsorption column is connected to a water removal column. The porous fiber according to the present invention has a three-dimensional pore structure formed by a solid fiber, and satisfies all of the following conditions. (1) The porous fiber has particulates having a diameter of not more than 200 μm, and the percentage of area occupied by said particulates having a diameter of not more than 200 μm in a horizontal cross section of the three-dimensional pore structure is at least 3.0%. (2) The porous fiber does not contain said particulates having a diameter of not more than 200 μm in the region within 1.0 μm in the depth direction from the outermost surface.

The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

A liquid chromatography packing material which includes a polymer packing material into which 1.50 mmol or more of carboxyl groups are introduced per 1 g of the packing material, and an index indicating pH of the surface of the polymer packing material as determined by hydrophilic interaction chromatography (HILIC) is 1.30 or more and the index indicating hydrophilicity of the surface of the polymer packing material as determined by a hydrophilic interaction chromatography (HILIC) is from 1.00 to 1.30.

A liquid chromatography packing material which includes a polymer packing material into which 1.50 mmol or more of carboxyl groups are introduced per 1 g of the packing material, and an index indicating pH of the surface of the polymer packing material as determined by hydrophilic interaction chromatography (HILIC) is 1.30 or more and the index indicating hydrophilicity of the surface of the polymer packing material as determined by a hydrophilic interaction chromatography (HILIC) is from 1.00 to 1.30.

The present invention provides a process for recovering a viral product from a composition comprising said product and product-related impurities, which process comprises contacting the composition with a functionalised chromatography medium comprising one or more polymer nanofibres, wherein the viral product comprises a plurality of viruses, virus particles/virions, viral cores, membrane-stripped viruses, viral cores with outer membrane(s) removed and/or capsids removed, or proviruses, each of which contains one or more polynucleotides, and wherein the product-related impurities comprise a plurality of viruses, virus particles/virions, virus-like particles, viral cores, membrane-stripped viruses, viral cores with outer membrane(s) removed and/or capsids removed or proviruses, each of which is substantially devoid of polynucleotides.

The present invention provides a process for recovering a viral product from a composition comprising said product and product-related impurities, which process comprises contacting the composition with a functionalised chromatography medium comprising one or more polymer nanofibres, wherein the viral product comprises a plurality of viruses, virus particles/virions, viral cores, membrane-stripped viruses, viral cores with outer membrane(s) removed and/or capsids removed, or proviruses, each of which contains one or more polynucleotides, and wherein the product-related impurities comprise a plurality of viruses, virus particles/virions, virus-like particles, viral cores, membrane-stripped viruses, viral cores with outer membrane(s) removed and/or capsids removed or proviruses, each of which is substantially devoid of polynucleotides.

The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.

The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.