An object of the present invention is to provide a separation medium and a separation method, ensuring high selectivity and good separation efficiency for specific steviol glycosides. The present invention is related to a separation medium in which polyethyleneimine is immobilized to porous particles of a (meth)acrylic polymer having a crosslinked structure and a hydroxyl group.

Provided is a stationary phase for chromatography, the stationary phase being made of inorganic carrier particles to which is bonded a polymer having a hydrophilic group on repeating units of a main chain thereof, and being produced by a particular production method.

Provided is a stationary phase for chromatography, the stationary phase being made of inorganic carrier particles to which is bonded a polymer having a hydrophilic group on repeating units of a main chain thereof, and being produced by a particular production method.

A packing material for ion chromatography has a structure in which a polyethyleneimine is bonded, directly or through a spacer, to a surface of an organic porous substrate constituted of a hydroxylated crosslinked copolymer, and a functional group represented by formula (1)

##STR00001##

(wherein the symbols are as described in the description) is bonded to a nitrogen atom derived from the polyethyleneimine. The invention further relates to a production method of the packing material for ion chromatography and a column for ion chromatography. A packing material is provided for a column which exhibits a high separating performance in anion chromatography employing a hydroxide-based eluent, and a production method thereof is also provided.

A packing material for ion chromatography has a structure in which a polyethyleneimine is bonded, directly or through a spacer, to a surface of an organic porous substrate constituted of a hydroxylated crosslinked copolymer, and a functional group represented by formula (1)

##STR00001##

(wherein the symbols are as described in the description) is bonded to a nitrogen atom derived from the polyethyleneimine. The invention further relates to a production method of the packing material for ion chromatography and a column for ion chromatography. A packing material is provided for a column which exhibits a high separating performance in anion chromatography employing a hydroxide-based eluent, and a production method thereof is also provided.

The present invention provides porous materials for use in solid phase extractions and chromatography. In particular, the materials exhibit superior properties in the SPE analysis of biological materials. In certain aspects, the porous materials comprise a copolymer of a least one hydrophobic monomer and at least one hydrophilic monomer, wherein more than 10% of the BJH surface area of the porous material is contributed by pores that have a diameter greater than or equal to 200 Å, wherein said material has a median pore diameter of about 100 Å to about 1000 Å, or both. In some embodiments, the at least one hydrophilic monomer has a log P value of less than 0.5. In some embodiments, the at least one hydrophilic monomer is selected from 4-acryloymorphine, N-(3-methoxypropyl)acrylamide, N,N′-methylenebis(acrylamide), acrylonitrile, ethylene glycol dimethacrylate, methyl acrylate, 4-acetoxystyrene, 4-vinyl pyridine, or a boronic-acid-containing monomer, among others.

The present invention provides porous materials for use in solid phase extractions and chromatography. In particular, the materials exhibit superior properties in the SPE analysis of biological materials. In certain aspects, the porous materials comprise a copolymer of a least one hydrophobic monomer and at least one hydrophilic monomer, wherein more than 10% of the BJH surface area of the porous material is contributed by pores that have a diameter greater than or equal to 200 Å, wherein said material has a median pore diameter of about 100 Å to about 1000 Å, or both. In some embodiments, the at least one hydrophilic monomer has a log P value of less than 0.5. In some embodiments, the at least one hydrophilic monomer is selected from 4-acryloymorphine, N-(3-methoxypropyl)acrylamide, N,N′-methylenebis(acrylamide), acrylonitrile, ethylene glycol dimethacrylate, methyl acrylate, 4-acetoxystyrene, 4-vinyl pyridine, or a boronic-acid-containing monomer, among others.

The present disclosure provides devices, systems, kits and methods useful for quantitation of biomolecules such as intact proteins and nucleic acids.

Porous hybrid inorganic/organic materials comprising ordered domains are disclosed. Methods of making the materials and use of the materials for chromatographic applications are also disclosed.

Porous hybrid inorganic/organic materials comprising ordered domains are disclosed. Methods of making the materials and use of the materials for chromatographic applications are also disclosed.