The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

A chromatography column for the use of separation of acidic sugar chains, wherein the column comprises a first column and a second column, the second column connected by a flow path downstream of an outlet of the first column, and selected from the following (1) or (2): (1) the carrier of the first column is hydrophobically modified silica having a group containing a primary amine, a secondary amine or/and a tertiary amine, and the carrier of the second column is a resin having a group containing a primary amine, a secondary amine or/and a tertiary amine; (2) the carrier of the first column is a resin having a group containing a primary amine, a secondary amine or/and a tertiary amine, and the carrier of the second column is hydrophobically modified silica having a group containing a primary amine, a secondary amine, or/and a tertiary amine.

A chromatography column for the use of separation of acidic sugar chains, wherein the column comprises a first column and a second column, the second column connected by a flow path downstream of an outlet of the first column, and selected from the following (1) or (2): (1) the carrier of the first column is hydrophobically modified silica having a group containing a primary amine, a secondary amine or/and a tertiary amine, and the carrier of the second column is a resin having a group containing a primary amine, a secondary amine or/and a tertiary amine; (2) the carrier of the first column is a resin having a group containing a primary amine, a secondary amine or/and a tertiary amine, and the carrier of the second column is hydrophobically modified silica having a group containing a primary amine, a secondary amine, or/and a tertiary amine.

A gas chromatography device for peak focusing of one or more target analytes is provided that include a chromatographic column with an inlet and an outlet. A stationary phase is deposited inside the chromatographic column and has a positive thickness gradient. The stationary phase extends from the inlet to the outlet and has a first thickness at the inlet of the chromatographic column and a second thickness at the outlet of the chromatographic column. The second thickness is at least about 10% greater than the first thickness. Methods of peak focusing in a gas chromatography device, method of verifying peak focusing in a gas chromatography device and creating a gas chromatography device having a chromatographic column with a positive thickness gradient are also provided.

A gas chromatography device for peak focusing of one or more target analytes is provided that include a chromatographic column with an inlet and an outlet. A stationary phase is deposited inside the chromatographic column and has a positive thickness gradient. The stationary phase extends from the inlet to the outlet and has a first thickness at the inlet of the chromatographic column and a second thickness at the outlet of the chromatographic column. The second thickness is at least about 10% greater than the first thickness. Methods of peak focusing in a gas chromatography device, method of verifying peak focusing in a gas chromatography device and creating a gas chromatography device having a chromatographic column with a positive thickness gradient are also provided.

A preparation method of a resin-based iron oxide-containing composite phosphate removal adsorbent is provided. An alkaline anion resin is taken as a base, a potassium ferrate is used as an iron source, and a characteristics of ferrate ions easily adsorbed on a surface of the anion resin are utilized to prepare resin-based iron oxide-containing composite phosphate removal adsorbent by one-step in-situ hydrolysis precipitation, compared with the related art, a preparation process of the disclosure is relatively simpler, a time period is shorter, and a production cost is lower. It has a strong ability to eliminate interference from other anions in the waste effluents, and it has a strong adsorption capacity, fast adsorption speed and large adsorption capacity for the phosphate. Moreover, it has the advantages of strong regeneration ability and multiple repeated use times.

A preparation method of a resin-based iron oxide-containing composite phosphate removal adsorbent is provided. An alkaline anion resin is taken as a base, a potassium ferrate is used as an iron source, and a characteristics of ferrate ions easily adsorbed on a surface of the anion resin are utilized to prepare resin-based iron oxide-containing composite phosphate removal adsorbent by one-step in-situ hydrolysis precipitation, compared with the related art, a preparation process of the disclosure is relatively simpler, a time period is shorter, and a production cost is lower. It has a strong ability to eliminate interference from other anions in the waste effluents, and it has a strong adsorption capacity, fast adsorption speed and large adsorption capacity for the phosphate. Moreover, it has the advantages of strong regeneration ability and multiple repeated use times.

The present disclosure features a composition, including molecularly imprinted crosslinked polymers that have been imprinted with trimethylamine N-oxide. The molecularly imprinted crosslinked polymers have specific binding sites for trimethylamine N-oxide, and a trimethylamine N-oxide absorption capacity of at least 0.5 mg/g.

The present technology relates to a novel sanitization method for chromatography media and supporting equipment comprising treatment with a sanitization/sterilization solution comprising acetic acid and hexylene glycol.

A polymer for liquid chromatography or solid phase extraction is provided. The polymer is prepared by polymerizing styrene and divinylbenzene to form a styrene-divinylbenzene copolymer; soaking the styrene-divinylbenzene copolymer in a swelling agent to form nano-scale micropores; and soaking the microporous styrene-divinylbenzene copolymer in methanol. When packed in a chromatographic column, the polymer can be used to produce produce natural health or medicinal products from Cannabis species, for example, industrial hemp.