Embodiments described herein relate to novel chromatography media for removing anti-A and/or anti-B antibodies from a sample, as well as methods of using the same. The media described herein have several advantages over previously described media including, acid and alkaline stability.

A chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing. The packing includes a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing. A continuous medium permeable to molecular diffusion extends between the ducts, including a porous organic gel or an organic liquid with at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated. The at least one species has a diffusive path between the ducts.

A chromatography method in which a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing. The packing includes a plurality of capillary ducts extending in the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing. A continuous medium permeable to molecular diffusion extends between the ducts, including a porous organic gel or an organic liquid with at least one network of connected pores, the size of which is greater than two times the molecular diameter of at least one species to be separated. The at least one species has a diffusive path between the ducts.

The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

The invention discloses a functionalised chromatography medium, comprising: i) at least one non-woven layer (10) of polymeric nano fibres (20) comprising a plurality of nanofibre-nano fibre fusion points (30); ii) a grafted polymer coating covering the polymeric nanofibres and the nanofibre-nanofibre fusion points; iii) a plurality of ligand groups covalently bound to the grafted polymer coating, wherein the ligand groups are capable of interacting with a target biomolecule.

The invention discloses a functionalised chromatography medium, comprising: i) at least one non-woven layer (10) of polymeric nano fibres (20) comprising a plurality of nanofibre-nano fibre fusion points (30); ii) a grafted polymer coating covering the polymeric nanofibres and the nanofibre-nanofibre fusion points; iii) a plurality of ligand groups covalently bound to the grafted polymer coating, wherein the ligand groups are capable of interacting with a target biomolecule.

The present invention relates to a method for measuring a polymer modification ratio, and more particularly, to a method for measuring a polymer modification ratio, which includes preparing a first solution by dissolving a polymer mixture containing a modified polymer and an unmodified polymer in a first solvent, injecting the first solution into a column filled with an adsorbent, adsorbing the modified polymer onto the adsorbent, and eluting the first solution in which the unmodified polymer is dissolved, transferring the eluted first solution to a detector, injecting a second solvent into the column to elute the second solution in which the adsorbed modified polymer is dissolved, and transferring the eluted second solution to the detector.

A polymer for liquid chromatography or solid phase extraction is provided. The polymer is prepared by polymerizing styrene and divinylbenzene to form a styrene-divinylbenzene copolymer; soaking the styrene-divinylbenzene copolymer in a swelling agent to form nano-scale micropores; and soaking the microporous styrene-divinylbenzene copolymer in methanol. When packed in a chromatographic column, the polymer can be used to produce produce natural health or medicinal products from Cannabis species, for example, industrial hemp.

A polymer for liquid chromatography or solid phase extraction is provided. The polymer is prepared by polymerizing styrene and divinylbenzene to form a styrene-divinylbenzene copolymer; soaking the styrene-divinylbenzene copolymer in a swelling agent to form nano-scale micropores; and soaking the microporous styrene-divinylbenzene copolymer in methanol. When packed in a chromatographic column, the polymer can be used to produce produce natural health or medicinal products from Cannabis species, for example, industrial hemp.

This invention employs columns and methods to apply external and internal standards and compounds. Analytical standard or compounds are adsorbed to a solid phase extraction media and are stored indefinitely. The standards or compounds remain stable on the solid phase extraction media without decomposing. The standards or compounds may be removed from the solid phase extraction media with a solvent.