The present disclosure provides novel methods to purify and quantify extracellular vesicles (EVs) in biological samples, e.g plasma or cerebrospinal fluid (CSF).

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

This invention employs columns and methods to apply external and internal standards and compounds. Analytical standard or compounds are adsorbed to a solid phase extraction media and are stored indefinitely. The standards or compounds remain stable on the solid phase extraction media without decomposing. The standards or compounds may be removed from the solid phase extraction media with a solvent.

One embodiment shows a method for producing porous cellulose particles, including: (a) dissolving cellulose diacetate into a solvent to prepare a cellulose diacetate solution; (b) dispersing the cellulose diacetate solution into a medium immiscible with the cellulose diacetate solution to obtain a dispersed system; (c) cooling the dispersed system; (d) adding a poor solvent to the cooled dispersed system to precipitate cellulose diacetate particles; and (e) saponifying the cellulose diacetate particles.

One embodiment shows a method for producing porous cellulose particles, including: (a) dissolving cellulose diacetate into a solvent to prepare a cellulose diacetate solution; (b) dispersing the cellulose diacetate solution into a medium immiscible with the cellulose diacetate solution to obtain a dispersed system; (c) cooling the dispersed system; (d) adding a poor solvent to the cooled dispersed system to precipitate cellulose diacetate particles; and (e) saponifying the cellulose diacetate particles.

A relatively fast, inexpensive, and non-destructive method for separation and isolation of biologically active nanoparticles is described. Methods include the use of solid phase separation medis such as channeled fibers in a hydrophobic interaction chromatography (HIC) protocol to isolate biologically active nanoparticles from other components of a mixture. Biologically active nanoparticles can include natural nanoparticles (e.g., exosomes, lysosomes, virus particles) as well as synthetic nanoparticles (liposomes, genetically modified virus particles, etc.)

As a technique for separating odorous compounds by using a supercritical fluid chromatograph, a method for separating an odorous compound is provided which includes a process of carrying a sample containing an odorous compound into a column filled with a packing material made of a polymer having an unsaturated hydrocarbon structure, by a flow of a mobile phase which is a supercritical fluid of a predetermined substance.

The present invention provides a separation material comprising porous polymer particles that comprise a styrene-based monomer as a monomer unit; and a coating layer that comprises a macromolecule having hydroxyl groups and covers at least a portion of the surface of the porous polymer particles, wherein the rupture strength is 10 mN or higher.

The present invention provides a separation material comprising porous polymer particles that comprise a styrene-based monomer as a monomer unit; and a coating layer that comprises a macromolecule having hydroxyl groups and covers at least a portion of the surface of the porous polymer particles, wherein the rupture strength is 10 mN or higher.