A material for reverse phase chromatography comprises surface modifying apolar and charged groups bound to a solid support, said charged groups being present in amounts of about 0.25 to about 22% of the surface modifying groups, or in amounts of about 0.01 mol/m.sup.2 to 0.8 mol/m.sup.2 referred to the surface of the solid support for a material with a total amount of surface modifying groups of 3.6 mol/m.sup.2. Such material and suitable purification conditions for active pharmaceutical ingredients (APIs) like peptides can be evaluated by (a) determining the isoelectric point (pI) of the API of interest, (b) choosing a pH in a range where the solid phase material is stable, (c) determining the difference pIpH and (d) if the difference pIpH is positive, choosing an anion exchange (AIEX) material, or if the difference pIpH is negative, choosing an cation exchange (CIEX) material.

A chromatographic stationary phase or solid phase extraction (SPE) sorbent are sol-gel metal oxide particles comprising metal oxide network units with organic functionality bonded to the metal of the metal oxide and possess a higher performance or capacity than that of conventional ligand coated silica particles. The organic functionality is distributed throughout the metal oxide particle and wherein the mole percent of metals of the metal oxides with bonded organic functionality is in excess of nine mole percent of the particle. The particles are prepared from sol-gel processing employing an acid catalyst or an acid catalyst followed by a base catalyst to metal oxide precursors, at least nine mole percent of which have organic functionality. The particles are processed from a sol-gel condensation product into a size depending on the intended use.

The present disclosure describes a device for generating ionized molecules for analysis in a mass spectrometer. The device includes: a mesh substrate coated with an extraction phase, the extraction phase comprising a polymer that absorbs a molecule of interest from a matrix, or a polymer and solid phase microextraction (SPME) particles having pores dimensioned to absorb a molecule of interest from a matrix, where the mesh substrate has a sufficiently open structure to allow fluid to flow through the mesh substrate; and a solid substrate connected to the mesh substrate to provide stability to the coated mesh substrate. Mass spectrometry systems that include such a device are also described. Methods of analyzing an analyte previously extracted from a matrix onto the device are also described.

The present disclosure describes a device for generating ionized molecules for analysis in a mass spectrometer. The device includes: a mesh substrate coated with an extraction phase, the extraction phase comprising a polymer that absorbs a molecule of interest from a matrix, or a polymer and solid phase microextraction (SPME) particles having pores dimensioned to absorb a molecule of interest from a matrix, where the mesh substrate has a sufficiently open structure to allow fluid to flow through the mesh substrate; and a solid substrate connected to the mesh substrate to provide stability to the coated mesh substrate. Mass spectrometry systems that include such a device are also described. Methods of analyzing an analyte previously extracted from a matrix onto the device are also described.

In the specification, a method for purifying a compound by column chromatography, and a method for optimizing the symmetry factor and theoretical plate number by allowing the slurry to settle and reducing the sedimentation volume of the slurry. A compound purified by such method is also provided.

In the specification, a method for purifying a compound by column chromatography, and a method for optimizing the symmetry factor and theoretical plate number by allowing the slurry to settle and reducing the sedimentation volume of the slurry. A compound purified by such method is also provided.

The present invention relates to a method for conditioning reversed phase SPE cartridges that provides certain advantages compared with known such methods. The method of the invention finds particular use in the automated synthesis of radiolabeled compounds where SPE is used for example in the purification steps.

The present invention relates to a method for conditioning reversed phase SPE cartridges that provides certain advantages compared with known such methods. The method of the invention finds particular use in the automated synthesis of radiolabeled compounds where SPE is used for example in the purification steps.

The present invention relates to development of stationary phases and preparation of extraction columns having substantially improved capacity (i.e., amount of material purified per single chromatographic run) for lab scale to industrial scale extraction chromatographic separation, from small scale to industrial scale, of rare earth elements (REEs) and the platinum group metals (PGMs). More specifically the invention relates to preparation of stationary phases and extraction columns for extraction of REEs or PGMs as a group from containing matrices of typical REE or PGM feedstock and separation and purification of individual REEs or PGMs from each other.

The present invention relates to development of stationary phases and preparation of extraction columns having substantially improved capacity (i.e., amount of material purified per single chromatographic run) for lab scale to industrial scale extraction chromatographic separation, from small scale to industrial scale, of rare earth elements (REEs) and the platinum group metals (PGMs). More specifically the invention relates to preparation of stationary phases and extraction columns for extraction of REEs or PGMs as a group from containing matrices of typical REE or PGM feedstock and separation and purification of individual REEs or PGMs from each other.