The present invention relates to a multimodal adsorption medium, in particular a multimodal chromatography medium, a method for its production, as well as use of the adsorption medium according to the invention or an adsorption medium produced according to the invention for the purification of biomolecules.

The present invention relates to a multimodal adsorption medium, in particular a multimodal chromatography medium, a method for its production, as well as use of the adsorption medium according to the invention or an adsorption medium produced according to the invention for the purification of biomolecules.

A chromatographic media for separating bio-polymers, the chromatographic media having cationic exchange properties and anionic exchange properties, the chromatographic media comprising: (a) non-porous substrate particles including an organic polymer, the substrate particles having a neutral hydrophilic layer at a surface of the non-porous substrate particles, in which the neutral hydrophilic layer is configured to reduce a binding of the bio-polymers directly to the non-porous substrate particles compared to a binding of the bio-polymer to the non-porous substrate particles without the neutral hydrophilic layer; (b) a charged first ion exchange layer bound to the substrate particles on top of the hydrophilic layer, the first ion exchange layer comprising first ion exchange groups; and (c) a charged second ion exchange layer bound to the substrate particles on top of the first ion exchange layer.

A chromatographic media for separating bio-polymers, the chromatographic media having cationic exchange properties and anionic exchange properties, the chromatographic media comprising: (a) non-porous substrate particles including an organic polymer, the substrate particles having a neutral hydrophilic layer at a surface of the non-porous substrate particles, in which the neutral hydrophilic layer is configured to reduce a binding of the bio-polymers directly to the non-porous substrate particles compared to a binding of the bio-polymer to the non-porous substrate particles without the neutral hydrophilic layer; (b) a charged first ion exchange layer bound to the substrate particles on top of the hydrophilic layer, the first ion exchange layer comprising first ion exchange groups; and (c) a charged second ion exchange layer bound to the substrate particles on top of the first ion exchange layer.

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W).sub.a(Q).sub.b(T).sub.c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05?(b/c)?100, and a?0.

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W).sub.a(Q).sub.b(T).sub.c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05?(b/c)?100, and a?0.

A material for reverse phase chromatography comprises surface modifying apolar and charged groups bound to a solid support, said charged groups being present in amounts of about 0.25 to about 22% of the surface modifying groups, or in amounts of about 0.01 mol/m.sup.2 to 0.8 mol/m.sup.2 referred to the surface of the solid support for a material with a total amount of surface modifying groups of 3.6 mol/m.sup.2. Such material and suitable purification conditions for active pharmaceutical ingredients (APIs) like peptides can be evaluated by (a) determining the isoelectric point (pI) of the API of interest, (b) choosing a pH in a range where the solid phase material is stable, (c) determining the difference pIpH and (d) if the difference pIpH is positive, choosing an anion exchange (AIEX) material, or if the difference pIpH is negative, choosing an cation exchange (CIEX) material.

A material for reverse phase chromatography comprises surface modifying apolar and charged groups bound to a solid support, said charged groups being present in amounts of about 0.25 to about 22% of the surface modifying groups, or in amounts of about 0.01 mol/m.sup.2 to 0.8 mol/m.sup.2 referred to the surface of the solid support for a material with a total amount of surface modifying groups of 3.6 mol/m.sup.2. Such material and suitable purification conditions for active pharmaceutical ingredients (APIs) like peptides can be evaluated by (a) determining the isoelectric point (pI) of the API of interest, (b) choosing a pH in a range where the solid phase material is stable, (c) determining the difference pIpH and (d) if the difference pIpH is positive, choosing an anion exchange (AIEX) material, or if the difference pIpH is negative, choosing an cation exchange (CIEX) material.

Provided is a stationary phase for supercritical fluid chromatography, the stationary phase having satisfactory molecule-identifying ability, in particular, satisfactory separating properties with respect to not only acidic compounds or basic compounds but also fused aromatic compounds or aromatic isomers. The stationary phase for supercritical fluid chromatography includes a support having, bonded thereto, a polymer in which the main chain has nitrogenous aromatic rings in the repeating units.

Provided are a technique for preparing a porous cellulose medium without using a special gelling agent for a solution in which cellulose acetate serving as a raw material is dissolved; and a porous cellulose medium and the like produced using the technique. A method for producing a porous cellulose medium comprises the step of preparing a flowable homogeneous composition comprising cellulose acetate, a basic compound, and a solvent including water, and gelling the composition by deacetylation reaction of the cellulose acetate.