Novel material for chromatographic separations, processes for its preparation, and separation devices containing the chromatographic material. In particular, the novel materials are porous silicon oxynitride materials, which desirably can be surface modified and have enhanced stability at high pH. The novel porous silicon oxynitride material may offer efficient chromatographic separations, and hold great promise as packing material for chromatographic separations.

Novel material for chromatographic separations, processes for its preparation, and separation devices containing the chromatographic material. In particular, the novel materials are porous silicon oxynitride materials, which desirably can be surface modified and have enhanced stability at high pH. The novel porous silicon oxynitride material may offer efficient chromatographic separations, and hold great promise as packing material for chromatographic separations.

Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)N(R.sup.1, R.sup.2)R.sup.3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R.sup.1 and R.sup.2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R.sup.3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR.sup.4, O, or S; wherein R.sup.4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.

Direct polymerization of lipid monomers or polymer scaffolding of non-lipid monomers coupled with irradiation or redox polymerization performed at neutral pH resulted in stabilized lipid assemblies. An initiator-buffer component and NaHS03 redox mixture polymerizes reactive lipid monomers at near neutral pH conditions to preserve functionality of reconstituted membrane proteins. Improved stability of black lipid membranes (BLMs) is attained by chemical cross-linking of polymerizable, hydrophobic and commercially available non-lipid monomers partitioned into the suspended lipid membranes, and by suspending the BLMs across low surface energy apertures. Substrate apertures having low surface energy modifiers with amphiphobic properties facilitated a reproducible formation of BLMs by promoting interactions between the lipid tail and the substrate material. In addition, polymeric lipid bilayer membranes were prepared by photochemical or redox initiated polymerization of polymerizable lipid monomers, and disposed onto supporting substrates for use in chromatography columns.

Direct polymerization of lipid monomers or polymer scaffolding of non-lipid monomers coupled with irradiation or redox polymerization performed at neutral pH resulted in stabilized lipid assemblies. An initiator-buffer component and NaHS03 redox mixture polymerizes reactive lipid monomers at near neutral pH conditions to preserve functionality of reconstituted membrane proteins. Improved stability of black lipid membranes (BLMs) is attained by chemical cross-linking of polymerizable, hydrophobic and commercially available non-lipid monomers partitioned into the suspended lipid membranes, and by suspending the BLMs across low surface energy apertures. Substrate apertures having low surface energy modifiers with amphiphobic properties facilitated a reproducible formation of BLMs by promoting interactions between the lipid tail and the substrate material. In addition, polymeric lipid bilayer membranes were prepared by photochemical or redox initiated polymerization of polymerizable lipid monomers, and disposed onto supporting substrates for use in chromatography columns.

A temperature responsive monolithic porous material is obtained that comprises a polymer having a hydration ability that changes in a temperature range of 0 to 80 C. and being immobilized to a surface of the porous material at a high density by binding an atom transfer radical polymerization initiator to a surface of the porous material, and inducing a growth reaction of a polymer, having a hydration ability that changes in a temperature range of 0 to 80 C., from the initiator using an atom transfer radical process under a presence of a catalyst.

A temperature responsive monolithic porous material is obtained that comprises a polymer having a hydration ability that changes in a temperature range of 0 to 80 C. and being immobilized to a surface of the porous material at a high density by binding an atom transfer radical polymerization initiator to a surface of the porous material, and inducing a growth reaction of a polymer, having a hydration ability that changes in a temperature range of 0 to 80 C., from the initiator using an atom transfer radical process under a presence of a catalyst.

A method for producing a protein adsorbent comprising a substrate and a molecular chain fixed on the surface of the substrate is disclosed. The method comprises, in this order: a dry-heat treatment step of heating a pretreatment adsorbent comprising the substrate and the molecular chain fixed on the surface of the substrate, in which the molecular chain contains a weak electrolytic ion-exchange group; and a wet-heat treatment step of heating the pretreatment adsorbent in a moistened state with a liquid or steam to obtain the protein adsorbent.

A method for producing a protein adsorbent comprising a substrate and a molecular chain fixed on the surface of the substrate is disclosed. The method comprises, in this order: a dry-heat treatment step of heating a pretreatment adsorbent comprising the substrate and the molecular chain fixed on the surface of the substrate, in which the molecular chain contains a weak electrolytic ion-exchange group; and a wet-heat treatment step of heating the pretreatment adsorbent in a moistened state with a liquid or steam to obtain the protein adsorbent.

Sample preparation and separation can be performed using a sample cartridge (201). The cartridge includes a barrel (204) with a first and second end, a column segment (209) connected to the second end of the barrel, and a column (205) containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.