Provided is a stationary phase for supercritical fluid chromatography, the stationary phase having satisfactory molecule-identifying ability, in particular, satisfactory separating properties with respect to not only acidic compounds or basic compounds but also fused aromatic compounds or aromatic isomers. The stationary phase for supercritical fluid chromatography includes a support having, bonded thereto, a polymer in which the main chain has nitrogenous aromatic rings in the repeating units.

Provided is a stationary phase for supercritical fluid chromatography, the stationary phase having satisfactory molecule-identifying ability, in particular, satisfactory separating properties with respect to not only acidic compounds or basic compounds but also fused aromatic compounds or aromatic isomers. The stationary phase for supercritical fluid chromatography includes a support having, bonded thereto, a polymer in which the main chain has nitrogenous aromatic rings in the repeating units.

The present disclosure discusses a method of separating a sample (e.g., peptide compound) including coating a flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating (e.g., organosilica coating) of the flow path. The applied coating can reduce peak tailing and increase analyte recovery for the sample of the chromatographic system.

Provided is a chromatography stationary phase having an excellent molecule discriminating ability. Specifically, provided is a chromatography stationary phase including a carrier carrying a copolymer that has a pyrrolidone backbone or a piperidone backbone, and an imide backbone in a repeating unit of the main chain.

Provided is a chromatography stationary phase having an excellent molecule discriminating ability. Specifically, provided is a chromatography stationary phase including a carrier carrying a copolymer that has a pyrrolidone backbone or a piperidone backbone, and an imide backbone in a repeating unit of the main chain.

The present invention relates to chromatography beads, production and use thereof. More closely the invention relates to small, rigid and nan-permeable agarose beads suitable for example as stationary phase in high performance liquid chromatography (HPLC) for analyses of biomolecules, such as, peptides and proteins; and methods for producing such beads.

The present invention relates to chromatography beads, production and use thereof. More closely the invention relates to small, rigid and nan-permeable agarose beads suitable for example as stationary phase in high performance liquid chromatography (HPLC) for analyses of biomolecules, such as, peptides and proteins; and methods for producing such beads.

Sample preparation and separation can be performed using a sample cartridge (201). The cartridge includes a barrel (204) with a first and second end, a column segment (209) connected to the second end of the barrel, and a column (205) containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.

Sample preparation and separation can be performed using a sample cartridge (201). The cartridge includes a barrel (204) with a first and second end, a column segment (209) connected to the second end of the barrel, and a column (205) containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.

Solid phase extraction (SPE) disks are manufactured by introducing a series of components and/or liquid suspensions into a mold and evacuating the liquid to form a cohesive filter or SPE disk. After all the free liquid has been substantially removed, the SPE disk is removed from the mold and dried. SPE disks are for use in analytical chemistry procedures.