In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W).sub.a(Q).sub.b(T).sub.c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 may be the same or different, and are chosen from H, halogen, C.sub.1-C.sub.4 alkyl, or C.sub.1-C.sub.4 alkoxy provided that at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4 or R.sub.5 is not hydrogen. ##STR00001##

The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 may be the same or different, and are chosen from H, halogen, C.sub.1-C.sub.4 alkyl, or C.sub.1-C.sub.4 alkoxy provided that at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4 or R.sub.5 is not hydrogen. ##STR00001##

The present invention provides a degradable microbead comprising a polymer molecule crosslinked by a crosslinking agent, wherein the polymer molecule and/or the crosslinking agent comprises a sensitive chemical bond that is cleavable through a chemical and/or light treatment, thereby resulting in the degradation of the degradable microbead. The present invention also provides a method of separating a target protein from a sample. By using the degradation of the degradable microbead to replace an elution step in protein purification, it is possible to select a combination of target protein and affinity ligand with a stronger affinity, thereby improving the protein purification efficiency. The method is especially suitable for the high-throughput preparation of multiple protein samples, for example providing a protein sample for electron microscope observation or mass spectrometry measurement.

Provided is a stationary phase for chromatography, the stationary phase being made of inorganic carrier particles to which is bonded a polymer having a hydrophilic group on repeating units of a main chain thereof, and being produced by a particular production method.

A packing material for ion chromatography has a structure in which a polyethyleneimine is bonded, directly or through a spacer, to a surface of an organic porous substrate constituted of a hydroxylated crosslinked copolymer, and a functional group represented by formula (1)

##STR00001##

(wherein the symbols are as described in the description) is bonded to a nitrogen atom derived from the polyethyleneimine. The invention further relates to a production method of the packing material for ion chromatography and a column for ion chromatography. A packing material is provided for a column which exhibits a high separating performance in anion chromatography employing a hydroxide-based eluent, and a production method thereof is also provided.

A packing material for ion chromatography has a structure in which a polyethyleneimine is bonded, directly or through a spacer, to a surface of an organic porous substrate constituted of a hydroxylated crosslinked copolymer, and a functional group represented by formula (1)

##STR00001##

(wherein the symbols are as described in the description) is bonded to a nitrogen atom derived from the polyethyleneimine. The invention further relates to a production method of the packing material for ion chromatography and a column for ion chromatography. A packing material is provided for a column which exhibits a high separating performance in anion chromatography employing a hydroxide-based eluent, and a production method thereof is also provided.

Porous hybrid inorganic/organic materials comprising ordered domains are disclosed. Methods of making the materials and use of the materials for chromatographic applications are also disclosed.

Provided is a chromatography stationary phase having an excellent molecule discriminating ability. Specifically, provided is a chromatography stationary phase including a carrier carrying a copolymer that has a pyrrolidone backbone or a piperidone backbone, and an imide backbone in a repeating unit of the main chain.

Provided is a chromatography stationary phase having an excellent molecule discriminating ability. Specifically, provided is a chromatography stationary phase including a carrier carrying a copolymer that has a pyrrolidone backbone or a piperidone backbone, and an imide backbone in a repeating unit of the main chain.