A method for producing a chromatography medium by: a) exposing a nanoweb sheet having mean flow pore size from 0.1 to 5 m and a porosity from 40 to 90 volume % to a gaseous phase comprising a vinyl monomer to produce a functionalized nanoweb sheet; b) layering a plurality of functionalized nanoweb sheets to form a functionalized nanoweb stack; c) cutting the functionalized nanoweb stack with a die to form die-cut functionalized nanoweb stacks having regular shapes; and d) exposing the die-cut functionalized nanoweb stacks in an aqueous medium to a ligand such as a protein which is capable of forming covalent bonds.

A method for producing a chromatography medium by: a) exposing a nanoweb sheet having mean flow pore size from 0.1 to 5 m and a porosity from 40 to 90 volume % to a gaseous phase comprising a vinyl monomer to produce a functionalized nanoweb sheet; b) layering a plurality of functionalized nanoweb sheets to form a functionalized nanoweb stack; c) cutting the functionalized nanoweb stack with a die to form die-cut functionalized nanoweb stacks having regular shapes; and d) exposing the die-cut functionalized nanoweb stacks in an aqueous medium to a ligand such as a protein which is capable of forming covalent bonds.

A nucleophilic substitution reaction to crosslink cyclodextrin (CD) polymer with rigid aromatic groups, providing a high surface area, mesoporous CD-containing polymers (P-CDPs). The P-CDPs can be used for removing organic contaminants from water. By encapsulating pollutants to form well-defined host-guest complexes with complementary selectivities to activated carbon (AC) sorbents. The P-CDPs can rapidly sequester pharmaceuticals, pesticides, and other organic micropollutants, achieving equilibrium binding capacity in seconds with adsorption rate constants 15-200 times greater than ACs and nonporous CD sorbents. The CD polymer can be regenerated several times, through a room temperature washing procedure, with no loss in performance.

A nucleophilic substitution reaction to crosslink cyclodextrin (CD) polymer with rigid aromatic groups, providing a high surface area, mesoporous CD-containing polymers (P-CDPs). The P-CDPs can be used for removing organic contaminants from water. By encapsulating pollutants to form well-defined host-guest complexes with complementary selectivities to activated carbon (AC) sorbents. The P-CDPs can rapidly sequester pharmaceuticals, pesticides, and other organic micropollutants, achieving equilibrium binding capacity in seconds with adsorption rate constants 15-200 times greater than ACs and nonporous CD sorbents. The CD polymer can be regenerated several times, through a room temperature washing procedure, with no loss in performance.

Embodiments described herein relate to novel chromatography media for removing anti-A and/or anti-B antibodies from a sample, as well as methods of using the same. The media described herein have several advantages over previously described media including, acid and alkaline stability.

Embodiments described herein relate to novel chromatography media for removing anti-A and/or anti-B antibodies from a sample, as well as methods of using the same. The media described herein have several advantages over previously described media including, acid and alkaline stability.

The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.

Provided herein are stationary phase compositions comprising a chromatographic surface of porous or non-porous core material comprising a surface modifier for use in chromatographic separations.

Provided herein are stationary phase compositions comprising a chromatographic surface of porous or non-porous core material comprising a surface modifier for use in chromatographic separations.

Composite material comprising a support material and at least one polymeric layer, wherein the at least one polymeric layer is present in form of a polymeric mesh and is comprising at least one non-adsorbing/non-adsorptive polymer with respect to a target compound, and wherein said composite material further comprises sites which are adsorbing/adsorptive for an impurity compound; and a combination of at least one first adsorbent and at least one second adsorbent, wherein the at least one first adsorbent comprises at least one composite material comprising at least one adsorbing/adsorptive polymer, or at least one non-adsorbing/non-adsorptive polymer, or at least one adsorbing/adsorptive polymer together with at least one non-adsorbing/non-adsorptive polymer.