The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.

A blood treatment material adsorbs and removes blood components such as activated leukocytes and inflammatory cytokines with a high efficiency. The blood treatment material includes a water-insoluble material in the form of fibers or particles, wherein the difference between the maximum value (RaA) and the minimum value (RaB) of the arithmetic average roughness (Ra) of the surface of the water-insoluble material, as calculated using a laser microscope, is from 0.30 to 1.50 μm.

A blood treatment material adsorbs and removes blood components such as activated leukocytes and inflammatory cytokines with a high efficiency. The blood treatment material includes a water-insoluble material in the form of fibers or particles, wherein the difference between the maximum value (RaA) and the minimum value (RaB) of the arithmetic average roughness (Ra) of the surface of the water-insoluble material, as calculated using a laser microscope, is from 0.30 to 1.50 μm.

In some aspects, the present disclosure pertains to chromatographic materials that comprise (a) a bulk material and (b) a zwitterionic polymer covalently linked to a surface of the bulk material, in which the zwitterionic polymer comprises one or more monomer residues that comprise an amide or urea moiety, a positively charged moiety, and a negatively charged moiety. Other aspects of the present disclosure pertain to chromatographic separation devices that comprise such chromatographic materials, to chromatographic methods that employ such chromatographic separation devices, and to kits that contain (i) such chromatographic materials and (ii) one or more chromatographic devices for containing such materials.

A smart sand includes raw sand particles, synthetic SiO.sub.2 particles attached to the raw sand particles, a first material attached to a first set of the synthetic SiO.sub.2 particles, a second material attached to a second set of the synthetic SiO.sub.2 particles, and a third material attached to the first material. Each of the first to third materials is different from each other.

A smart sand includes raw sand particles, synthetic SiO.sub.2 particles attached to the raw sand particles, a first material attached to a first set of the synthetic SiO.sub.2 particles, a second material attached to a second set of the synthetic SiO.sub.2 particles, and a third material attached to the first material. Each of the first to third materials is different from each other.

A nucleophilic substitution reaction to crosslink cyclodextrin (CD) polymer with rigid aromatic groups, providing a high surface area, mesoporous CD-containing polymers (P-CDPs). The P-CDPs can be used for removing organic contaminants from water. By encapsulating pollutants to form well-defined host-guest complexes with complementary selectivities to activated carbon (AC) sorbents. The P-CDPs can rapidly sequester pharmaceuticals, pesticides, and other organic micropollutants, achieving equilibrium binding capacity in seconds with adsorption rate constants 15-200 times greater than ACs and nonporous CD sorbents. The CD polymer can be regenerated several times, through a room temperature washing procedure, with no loss in performance.

A nucleophilic substitution reaction to crosslink cyclodextrin (CD) polymer with rigid aromatic groups, providing a high surface area, mesoporous CD-containing polymers (P-CDPs). The P-CDPs can be used for removing organic contaminants from water. By encapsulating pollutants to form well-defined host-guest complexes with complementary selectivities to activated carbon (AC) sorbents. The P-CDPs can rapidly sequester pharmaceuticals, pesticides, and other organic micropollutants, achieving equilibrium binding capacity in seconds with adsorption rate constants 15-200 times greater than ACs and nonporous CD sorbents. The CD polymer can be regenerated several times, through a room temperature washing procedure, with no loss in performance.

In various embodiments, the present disclosure pertains to core-shell particles that comprise a porous hybrid organic-inorganic shell disposed on a surface-modified non-porous polymer particle core. In some embodiments, the present disclosure pertains to chromatographic separation devices that comprise such core-shell particles. In some embodiments, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such core-shell particles and (b) flowing a mobile phase through the column.

Chromatography devices contain chromatography media and methods of making and methods of using chromatography devices. Chromatography devices enable a more efficient, productive and/or environmentally friendly chromatographic operation due to one or more of the following advantages over conventional chromatographic operations: elimination of a device packing step by the user; elimination of clean-in-place (CIP) steps; elimination of clean-in-place (CIP) steps utilizing sodium hydroxide solution; elimination of any validation steps by the user; and use of a chromatography device comprising biodegradable material. The chromatography media includes porous inorganic particles having a functionalized surface and having a median pore size of at least about 300 Angstroms (A), or at least about 300 A up to about 3000 A. The inorganic particles may have a BET surface area of at least about 20 m2/g, or at least about 25 m2/g, or about 30 m2/g, up to about 2000 m2/g.