Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing the ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).

Ligand functionalized substrate including a solid substrate, which has been modified to provide grafted catching ligand groups covalently bound via a linker, methods of preparing the ligand functionalized substrate and the use thereof, such as to increase binding rate and the dynamic binding capacity (DBC).

The present invention relates to the use, for affinity purification of an antibody or an fragment of an antibody, of a ligand-substituted matrix comprising a support material and at least one ligand covalently bonded to the support material, the ligand being represented by formula (I)
L-(Sp).sub.v-Ar.sup.1—Am—Ar.sup.2   (I)
wherein L, SP, Ar.sup.1, AM, Ar.sup.2 and v are defined herein.

The present invention relates to the use, for affinity purification of an antibody or an fragment of an antibody, of a ligand-substituted matrix comprising a support material and at least one ligand covalently bonded to the support material, the ligand being represented by formula (I)
L-(Sp).sub.v-Ar.sup.1—Am—Ar.sup.2   (I)
wherein L, SP, Ar.sup.1, AM, Ar.sup.2 and v are defined herein.

The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-droxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.

The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-droxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.

A stationary phase for solid-phase microextraction (SPME) devices is based on nickel and titanium alloy nuclei and a metal-organic framework (MOF) exterior, which may be used for chromatographic analysis in environmental, food, etc. applications. The method of preparation of the stationary phases includes a number of steps which provide a covalent adhesion of the MOF to the nickel/titanium alloy. In these stationary phases, the metal-organic framework is the only component that comes into contact with the sample to be analysed. The interior of the stationary phase is executed in nitinol and endows the system with thermal and mechanical stability this being the first time that it is used to support a metal-organic framework, and presenting extractive advantages in comparison with commercial SPME stationary phases.

A filtration material including a silica base material having a group represented by the following general formula (a0-1) [in formula (a0-1), Ya.sup.01 represents a divalent linking group; Ra.sup.01 represents a hydrocarbon group which may have a substituent; Ra.sup.02 represents a hydroxyl group or a hydrocarbon group having 1 to 6 carbon atoms which may have a substituent; n.sup.01 represents an integer of 0 to 5; and the symbol “*” represents a valence bond with respect to the silica base material].

##STR00001##

A filtration material including a silica base material having a group represented by the following general formula (a0-1) [in formula (a0-1), Ya.sup.01 represents a divalent linking group; Ra.sup.01 represents a hydrocarbon group which may have a substituent; Ra.sup.02 represents a hydroxyl group or a hydrocarbon group having 1 to 6 carbon atoms which may have a substituent; n.sup.01 represents an integer of 0 to 5; and the symbol “*” represents a valence bond with respect to the silica base material].

##STR00001##

Provided herein are stationary phase compositions comprising a chromatographic surface of porous or non-porous core material comprising a surface modifier for use in chromatographic separations.