An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.

A biosensor for single cell analysis is disclosed. The biosensor includes a substrate, an array of electrodes, and a passivation layer. The substrate includes a roughened surface, where the array of electrodes is patterned on the roughened surface. Each electrode includes a distal tip and a proximal end. The passivation layer is deposited on top of the biosensor and includes a microwell around the distal tip of an electrode. A single cell is trapped within the microwell and adhered onto the distal tip of the electrode for further single cell analysis.

An exemplary embodiment of the present disclosure provides a system for detecting antimicrobial resistance of a bacteria in a biological sample. In some embodiments, the system can include a plurality of containers, a detecting agent in each of the plurality of containers, and an antimicrobial agent in at least a portion of the plurality of containers. The antimicrobial agent is disposed in at least one of the plurality of containers. Each of the containers can contain at least a portion of the biological sample. The detecting agent can be configured to produce optically detectable changes responsive to bacterial respiration or growth, directly from patient samples of from patient sample cultures.

Three-dimensional cellular arrays, methods of making three-dimensional cellular arrays, and methods of identifying agents using the arrays are disclosed.

The disclosure relates to the fabrication of a three dimensional [3-D] cell culture membrane comprising one or more functionalized surfaces adapted to provide cell culture conditions suitable for the analysis of proliferation, differentiation or function of cells, typically eukaryotic or prokaryotic cells.

There is provided plate equipped with a mechanism to facilitate determination of the amount of a fluid added to or removed from a well in the plate. Other embodiments are also described.

An assay plate is provided. The assay plate has a body with a plurality of reservoirs formed therein. The reservoirs are shaped and aligned in the body in an orientation to induce drainage of fluids contained therein in a desired direction. A plate array and a funnel array forming an assembly for pooling of samples contained in the assay plate is also provided.

Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

A liquid biopsy system and method for the detection of biomarkers in bodily fluids is described. In particular, the system is suitable for detecting biomarkers of lung cancer in a subject.

Disclosed herein are multiwell plates suitable for spectrophotometry for low-volume liquid samples. For the multiwell plates, the bottom of at least one well has a layer of porous matrix disposed thereon, or is comprised of a layer of porous matrix. The layer of porous matrix permits a low-volume liquid sample to distribute evenly across the porous matrix. Also disclosed herein are methods of performing a photometric or spectrophotometric measurement on a liquid sample having a small volume, by using a multiwell plate comprising a layer of porous matrix.