Examples herein provide a device. The device includes a sample delivery component, which includes: a reagent chamber to contain at least one reagent; a sample chamber to contain a fluid sample; and a delivery channel extending from the reagent chamber and in fluid communication with the sample chamber and an output port, wherein the delivery channel is conducive mixing the at least one reagent and the fluid sample to form a mixture before the mixture reaches the output port and be discharged therefrom. The device includes a testing cassette detachable from the delivery component, which includes: an input port in fluid communication with a microfluidic reservoir, the input port to receive the discharged fluid sample from the output port; and a micro-fabricated integrated sensor in a microfluidic channel extending from the microfluidic reservoir.

A digital microfluidic (DMF) system, DMF cartridge, and method including integrated refractive index (RI) sensing is disclosed. The digital microfluidic DMF system and DMF cartridge may include, for example, a RI sensor (or sensor surface) directly in the droplet operations gap of a DMF cartridge. The digital microfluidic DMF system may include, for example, the DMF cartridge, one or more illumination sources, one or more optical measurement devices, and a controller. Additionally, a method of using the DMF system and DMF cartridge that includes integrated RI sensing is provided.

Present invention is related to a tumor microenvironment on chip or a biochip for cell therapy having a carrier, a first cell or tissue culture area and a second cell or tissue area imbedded within the carrier. The present invention provides a biochip successfully cooperating micro fluidic technology and cell culture achieving the goal for detecting or testing the function of cell therapy for cancer or tumor.

A portable diagnostic device for performing the diagnosis of pathogens, such as viruses, bacteria, microorganisms, etc., by rapidly detecting their nucleic acids in a biological sample to be tested. Also, the use of the portable diagnostic device and the methods for detection of at least one nucleic acid sequence of interest implemented with the aid of the portable diagnostic device.

The present invention is directed to methods and devices capable of target analyte separation and analysis, in particular highly sensitive separation and detection and free-solution analyte detection assays.

Provided is a method including encapsulating a biological material in a droplet having a volume of 500 nl or less, depositing the droplet to an addressable location of a substrate, and performing mass spectroscopy on the droplet. The method can further include conducting omic analysis on the droplet, such as sequencing DNA or analyzing mRNA, after the mass spectroscopy. In some cases, the method can be used to screen thousands of genetically different cells to identify correlations between genetics and the production of a metabolite, wherein the metabolite is detected by mass spectroscopy. Also provided is a system for performing the method.

The present invention provides devices and methods for generating a pulsatile fluid flow in a microchannel by means of external actuation of a thin flexible film. With the devices described herein, cycles of positive and negative actuation can be used to infuse or withdrawal fluid in a microchannel. Fluid can be recirculated over one or more microfluidic feature, such as a chemical or molecular receptor, biosensor, electrode, cell or biological material, chromatography feature, mixer, etc., in a way that would represent an advantage over the single-pass flow techniques common to most microfluidic devices. The devices and methods are particularly useful in vitro diagnostics (IVD), analytical chemistry, chromatography, and mixing applications in a variety of fields.

Systems and techniques are described for capturing target extracellular vesicles from a fluid sample. In some implementations, a microfluidic device includes a microfluidic channel where an internal surface of at least one wall of the microfluidic channel includes a plurality of grooves or ridges, or both grooves and ridges, arranged and configured to generate chaotic mixing within a fluid sample flowing through the microfluidic channel. The microfluidic device also includes a plurality of elongate flexible linker molecules, each having a molecular weight between about 1.8-4.8 kDa, where each elongate flexible linker molecule is bound at a first end to an internal surface of at least one wall of the microfluidic channel and is bound at a second end to one or more binding moieties that specifically bind to a target extracellular vesicle.

An analysis unit for quantitating detection target substances bound to antibodies includes wells and inclination parts. The wells each have a hole-like shape defined by an opening, an inner circumferential surface, and a bottom. The inclination parts each have an inclined surface connected to the inner circumferential surface and inclined downward such that whose height with respect to the bottom decreases as a distance from an outer circumferential side of the well increases.

The present invention relates to methods and systems for testing for the presence of a material such as one or more analyte types within a sample and more particularly, for improved single enzyme-linked immunosorbent assay (sELISA) testing as well as other variants of single-enzyme linked molecular analysis (SELMA).